Is a 67-kD Cytokinin-Binding Protein from Barley and Arabidopsis thaliana Leaves Involved in the Leaf Responses to Phenylurea Derivatives? (A Review)

Is a 67-kD Cytokinin-Binding Protein from Barley and Arabidopsis thaliana Leaves Involved in the... Cytokinin-binding proteins (67-kD CBP) were isolated from barley first leaves of 8–10-day-old plants and Arabidopsis thaliana rosette leaves of 9-week-old plants. A capability of these proteins for cytokinin binding was assessed from trans-zeatin competition with antiidiotypic antibodies (ABa-i) for complex formation with immobilized 67-kD CBP under competitive ELISA conditions. ABa-i were obtained against anti-trans-zeatin antibody. 67-kD CBP from A. thaliana and barley leaves, in combination with trans-zeatin, activated transcriptional elongation in the in vitro system containing chromatin and RNA polymerase I from the same leaves. A phenylurea derivative demonstrating a high cytokinin activity, N-phenyl-N1-(2-chloro-4-pyridyl)urea (4-PU-30) could not displace ABa-i from its complex with 67-kD CBP from either barley or A. thaliana leaves, which indicates the absence of its interaction with CBP. In the presence of 67-kD CBP, 4-PU-30 did not activate transcription in the in vitro system containing chromatin and RNA polymerase I from A. thaliana or barley leaves. This means that, as distinct from trans-zeatin, 4-PU-30 did not use 67-kD CBP for transcription activation. A. thaliana leaf preincubation in the 4-PU-30 solution for 1 h enhanced RNA synthesis in the transcriptional system containing chromatin and RNA polymerase I from these pretreated leaves. Thus, leaves recognized the 4-PU-30 signal using another receptor. 4-PU-30 capacity to retard leaf senescence also supports this supposition. Another highly active phenylurea derivative thidiazuron also enhanced RNA synthesis in leaves and retarded their senescence. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Is a 67-kD Cytokinin-Binding Protein from Barley and Arabidopsis thaliana Leaves Involved in the Leaf Responses to Phenylurea Derivatives? (A Review)

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Publisher
Springer Journals
Copyright
Copyright © 2004 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/B:RUPP.0000047828.61196.f5
Publisher site
See Article on Publisher Site

Abstract

Cytokinin-binding proteins (67-kD CBP) were isolated from barley first leaves of 8–10-day-old plants and Arabidopsis thaliana rosette leaves of 9-week-old plants. A capability of these proteins for cytokinin binding was assessed from trans-zeatin competition with antiidiotypic antibodies (ABa-i) for complex formation with immobilized 67-kD CBP under competitive ELISA conditions. ABa-i were obtained against anti-trans-zeatin antibody. 67-kD CBP from A. thaliana and barley leaves, in combination with trans-zeatin, activated transcriptional elongation in the in vitro system containing chromatin and RNA polymerase I from the same leaves. A phenylurea derivative demonstrating a high cytokinin activity, N-phenyl-N1-(2-chloro-4-pyridyl)urea (4-PU-30) could not displace ABa-i from its complex with 67-kD CBP from either barley or A. thaliana leaves, which indicates the absence of its interaction with CBP. In the presence of 67-kD CBP, 4-PU-30 did not activate transcription in the in vitro system containing chromatin and RNA polymerase I from A. thaliana or barley leaves. This means that, as distinct from trans-zeatin, 4-PU-30 did not use 67-kD CBP for transcription activation. A. thaliana leaf preincubation in the 4-PU-30 solution for 1 h enhanced RNA synthesis in the transcriptional system containing chromatin and RNA polymerase I from these pretreated leaves. Thus, leaves recognized the 4-PU-30 signal using another receptor. 4-PU-30 capacity to retard leaf senescence also supports this supposition. Another highly active phenylurea derivative thidiazuron also enhanced RNA synthesis in leaves and retarded their senescence.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Dec 23, 2004

References

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