Iron release from ferritin induced by light and ionizing radiation

Iron release from ferritin induced by light and ionizing radiation The reductive release of iron from ferritin by UV light or ionizing radiation has been investigated in separate experiments. When ferritin is exposed to light, the mineral core is the main photoreceptor for the Fe(III) reduction. In radiolytic studies, we determined that, in the absence of oxygen, the hydrated electron (e aq − ) is the reducing agent triggering redox reactions associated with iron mobilization from ferritin. In an aerobic system, the superoxide radical anion (O 2 •− ) is also involved in the iron release process. We found that, in photochemical and radiolytical studies, Fe(II) mobilization from ferritin required an iron chelator. Without a chelator, ferritin is an electron-storage molecule for a long period, on the order of at least several hours. The reductant or chelator entry into the ferritin core is not necessary for iron release. The ferrozine is a convenient chelating agent to monitor Fe(II) mobilization, due to a high extinction coefficient of $$ {\text{Fe}}\,\left( {\text{ferrozine}} \right)_{3}^{4 - } $$ and a high rate constant of complexation process (2.65 × 104 dm3 mol−1 s−1). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Iron release from ferritin induced by light and ionizing radiation

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Publisher
Springer Netherlands
Copyright
Copyright © 2010 by The Author(s)
Subject
Chemistry; Inorganic Chemistry ; Physical Chemistry ; Catalysis
ISSN
0922-6168
eISSN
1568-5675
D.O.I.
10.1007/s11164-010-0155-0
Publisher site
See Article on Publisher Site

Abstract

The reductive release of iron from ferritin by UV light or ionizing radiation has been investigated in separate experiments. When ferritin is exposed to light, the mineral core is the main photoreceptor for the Fe(III) reduction. In radiolytic studies, we determined that, in the absence of oxygen, the hydrated electron (e aq − ) is the reducing agent triggering redox reactions associated with iron mobilization from ferritin. In an aerobic system, the superoxide radical anion (O 2 •− ) is also involved in the iron release process. We found that, in photochemical and radiolytical studies, Fe(II) mobilization from ferritin required an iron chelator. Without a chelator, ferritin is an electron-storage molecule for a long period, on the order of at least several hours. The reductant or chelator entry into the ferritin core is not necessary for iron release. The ferrozine is a convenient chelating agent to monitor Fe(II) mobilization, due to a high extinction coefficient of $$ {\text{Fe}}\,\left( {\text{ferrozine}} \right)_{3}^{4 - } $$ and a high rate constant of complexation process (2.65 × 104 dm3 mol−1 s−1).

Journal

Research on Chemical IntermediatesSpringer Journals

Published: Aug 17, 2010

References

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