Involvement of dsRNA virus in the protein compositionand growth kinetics of host Trichomonas vaginalis

Involvement of dsRNA virus in the protein compositionand growth kinetics of host Trichomonas... Trichomonas vaginalis harbors a double-stranded (ds)-RNA virus, and the presence of virus is related to upregulated expression and phenotypic variation of a prominent immunogen (Khoshnan A, Alderete JF (1994) J Virol 68: 4 035–4 038). To further test the influence of virus on T. vaginalis , virus-infected (V + ) isolates were compared to virus-free (V - ), agar-cloned progeny trichomonads derived from the parental isolates for accumulation of total proteins and cysteine proteinases. Comparative high resolution two dimensional (2D)-SDS-PAGE was performed of trichomonads grown in a chemostat under identical conditions. At least 47 proteins were identified as specifically expressed by representative V + isolate 347, and ∼41 spots were specific to the corresponding V - progeny, showing an association between virus and the presence and absence of parasite proteins. Qualitatively and quantitatively dissimilar cysteine proteinase patterns were detected from numerous V + isolates and the V - progeny. A 2D analysis for isolate 347 showed the appearance of unique proteinase activities for parental parasites and presence of at least one proteinase in the V - progeny. Finally, the V + T. vaginalis isolate 347, but not the V - isolate 347 progeny nor other V + isolates, underwent fluctuations in density during chemostat growth allowing for purification of virus particles from the V + isolate 347 supernatants during decreased parasite density. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Involvement of dsRNA virus in the protein compositionand growth kinetics of host Trichomonas vaginalis

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Publisher
Springer Journals
Copyright
Copyright © Wien by 1997 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050130
Publisher site
See Article on Publisher Site

Abstract

Trichomonas vaginalis harbors a double-stranded (ds)-RNA virus, and the presence of virus is related to upregulated expression and phenotypic variation of a prominent immunogen (Khoshnan A, Alderete JF (1994) J Virol 68: 4 035–4 038). To further test the influence of virus on T. vaginalis , virus-infected (V + ) isolates were compared to virus-free (V - ), agar-cloned progeny trichomonads derived from the parental isolates for accumulation of total proteins and cysteine proteinases. Comparative high resolution two dimensional (2D)-SDS-PAGE was performed of trichomonads grown in a chemostat under identical conditions. At least 47 proteins were identified as specifically expressed by representative V + isolate 347, and ∼41 spots were specific to the corresponding V - progeny, showing an association between virus and the presence and absence of parasite proteins. Qualitatively and quantitatively dissimilar cysteine proteinase patterns were detected from numerous V + isolates and the V - progeny. A 2D analysis for isolate 347 showed the appearance of unique proteinase activities for parental parasites and presence of at least one proteinase in the V - progeny. Finally, the V + T. vaginalis isolate 347, but not the V - isolate 347 progeny nor other V + isolates, underwent fluctuations in density during chemostat growth allowing for purification of virus particles from the V + isolate 347 supernatants during decreased parasite density.

Journal

Archives of VirologySpringer Journals

Published: May 1, 1997

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