Investigations on the RNA binding and phosphorylation of groundnut bud necrosis virus nucleocapsid protein

Investigations on the RNA binding and phosphorylation of groundnut bud necrosis virus... Groundnut bud necrosis virus belongs to the genus Tospovirus , infects a wide range of crop plants and causes severe losses. To understand the role of the nucleocapsid protein in the viral life cycle, the protein was overexpressed in E. coli and purified by Ni-NTA chromatography. The purified N protein was well folded and was predominantly alpha-helical. Deletion analysis revealed that the C-terminal unfolded region of the N protein was involved in RNA binding. Furthermore, the N protein could be phosphorylated in vitro by Nicotiana benthamiana plant sap and by purified recombinant kinases such as protein kinase CK2 and calcium-dependent protein kinase. This is the first report of phoshphorylation of a nucleocapsid protein in the family Bunyaviridae . The possible implications of the present findings for the viral life cycle are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Investigations on the RNA binding and phosphorylation of groundnut bud necrosis virus nucleocapsid protein

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Publisher
Springer Vienna
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Biomedicine; Virology; Infectious Diseases; Medical Microbiology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-011-1110-0
Publisher site
See Article on Publisher Site

Abstract

Groundnut bud necrosis virus belongs to the genus Tospovirus , infects a wide range of crop plants and causes severe losses. To understand the role of the nucleocapsid protein in the viral life cycle, the protein was overexpressed in E. coli and purified by Ni-NTA chromatography. The purified N protein was well folded and was predominantly alpha-helical. Deletion analysis revealed that the C-terminal unfolded region of the N protein was involved in RNA binding. Furthermore, the N protein could be phosphorylated in vitro by Nicotiana benthamiana plant sap and by purified recombinant kinases such as protein kinase CK2 and calcium-dependent protein kinase. This is the first report of phoshphorylation of a nucleocapsid protein in the family Bunyaviridae . The possible implications of the present findings for the viral life cycle are discussed.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2011

References

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