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- Publisher
- Springer Journals
- Copyright
- Copyright © 2018 by American Society for Mass Spectrometry
- Subject
- Chemistry; Analytical Chemistry; Biotechnology; Organic Chemistry; Proteomics; Bioinformatics
- ISSN
- 1044-0305
- eISSN
- 1879-1123
- DOI
- 10.1007/s13361-018-1983-1
- Publisher site
- See Article on Publisher Site
Abstract
Analysis of nicotinamide adenine dinucleotide (NAD+/NADH)-modified electrodes is important for in vitro monitoring of key biological processes. In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to analyze NAD+/NADH-modified gold electrodes. Interestingly, no obvious characteristic peaks of nicotinamide fragment could be observed in the mass spectra of NAD+/NADH in their neutral sodium pyrophosphate form. However, after acidification, the characteristic peaks for both NAD+ and NADH were detected. This was due to the suppression effect of inner pyrophosphoric salts in both neutral molecules. Besides, it was proved that the suppression by inner salt was intramolecular. No obvious suppression was found between neighboring molecules. These results demonstrated the suppression effect of inner salts in ToF-SIMS analysis, providing useful evidence for the study of ToF-SIMS ionization mechanism of organic molecule-modified electrodes.
Journal
Journal of the American Society for Mass Spectrometry – Springer Journals
Published: Jun 4, 2018