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Investigation of the Ionization Mechanism of NAD+/NADH-Modified Gold Electrodes in ToF-SIMS Analysis

Investigation of the Ionization Mechanism of NAD+/NADH-Modified Gold Electrodes in ToF-SIMS Analysis Analysis of nicotinamide adenine dinucleotide (NAD+/NADH)-modified electrodes is important for in vitro monitoring of key biological processes. In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to analyze NAD+/NADH-modified gold electrodes. Interestingly, no obvious characteristic peaks of nicotinamide fragment could be observed in the mass spectra of NAD+/NADH in their neutral sodium pyrophosphate form. However, after acidification, the characteristic peaks for both NAD+ and NADH were detected. This was due to the suppression effect of inner pyrophosphoric salts in both neutral molecules. Besides, it was proved that the suppression by inner salt was intramolecular. No obvious suppression was found between neighboring molecules. These results demonstrated the suppression effect of inner salts in ToF-SIMS analysis, providing useful evidence for the study of ToF-SIMS ionization mechanism of organic molecule-modified electrodes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of The American Society for Mass Spectrometry Springer Journals

Investigation of the Ionization Mechanism of NAD+/NADH-Modified Gold Electrodes in ToF-SIMS Analysis

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Publisher
Springer Journals
Copyright
Copyright © 2018 by American Society for Mass Spectrometry
Subject
Chemistry; Analytical Chemistry; Biotechnology; Organic Chemistry; Proteomics; Bioinformatics
ISSN
1044-0305
eISSN
1879-1123
DOI
10.1007/s13361-018-1983-1
Publisher site
See Article on Publisher Site

Abstract

Analysis of nicotinamide adenine dinucleotide (NAD+/NADH)-modified electrodes is important for in vitro monitoring of key biological processes. In this work, time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to analyze NAD+/NADH-modified gold electrodes. Interestingly, no obvious characteristic peaks of nicotinamide fragment could be observed in the mass spectra of NAD+/NADH in their neutral sodium pyrophosphate form. However, after acidification, the characteristic peaks for both NAD+ and NADH were detected. This was due to the suppression effect of inner pyrophosphoric salts in both neutral molecules. Besides, it was proved that the suppression by inner salt was intramolecular. No obvious suppression was found between neighboring molecules. These results demonstrated the suppression effect of inner salts in ToF-SIMS analysis, providing useful evidence for the study of ToF-SIMS ionization mechanism of organic molecule-modified electrodes.

Journal

Journal of The American Society for Mass Spectrometry – Springer Journals

Published: Jun 4, 2018

References

  • In vivo NAD assay reveals the intracellular NAD contents and redox state in healthy human brain and their age dependences
    Zhu, X-H; Lu, M; Lee, B-Y; Ugurbil, K; Chen, W
  • Structure of the V. cholerae Na+−pumping NADH:quinone oxidoreductase
    Steuber, J; Vohl, G; Casutt, MS; Vorburger, T; Diederichs, K; Fritz, G
  • DT-Diaphorase as a bifunctional enzyme label that allows rapid enzymatic amplification and electrochemical redox cycling
    Kang, C; Kang, J; Lee, N-S; Yoon, YH; Yang, H
  • Mimicking horseradish peroxidase and NADH peroxidase by heterogeneous Cu2+-modified graphene oxide nanoparticles
    Wang, S; Cazelles, R; Liao, W-C; Vázquez-González, M; Zoabi, A; Abu-Reziq, R; Willner, I
  • Mechanistic insights into voltage-driven biocatalysis of a cytochrome P450 bactosomal film on a self-assembled monolayer
    Nerimetla, R; Walgama, C; Singh, V; Hartson, SD; Krishnan, S
  • Reversible redox of NADH and NAD+ at a hybrid lipid bilayer membrane using ubiquinone
    Ma, W; Li, D-W; Sutherland, TC; Li, Y; Long, Y-T; Chen, H-Y
  • Donor/acceptor-modified electrodes for photoelectrochemical and photobioelectrochemical applications
    Metzger, TS; Chandaluri, CG; Tel-Vered, R; Shenhar, R; Willner, I
  • Bioelectrocatalytic NAD+/NADH inter-conversion: transformation of an enzymatic fuel cell into an enzymatic redox flow battery
    Quah, T; Milton, RD; Abdellaoui, S; Minteer, SD
  • Direct visualization of initial SEI morphology and growth kinetics during lithium deposition by in situ electrochemical transmission electron microscopy
    Sacci, RL; Dudney, NJ; More, KL; Parent, LR; Arslan, I; Browning, ND; Unocic, RR
  • Detection of subsurface structures underneath dendrites formed on cycled lithium metal electrodes
    Harry, KJ; Hallinan, DT; Parkinson, DY; MacDowell, AA; Balsara, NP
  • In situ atomic force microscopy study of initial solid electrolyte interphase formation on silicon electrodes for Li-ion batteries
    Tokranov, A; Sheldon, BW; Li, C; Minne, S; Xiao, X
  • Atomic force microscopy studies on molybdenum disulfide flakes as sodium-ion anodes
    Lacey, SD; Wan, J; Cresce, A v W; Russell, SM; Dai, J; Bao, W; Xu, K; Hu, L
  • Improving lithium–sulphur batteries through spatial control of sulphur species deposition on a hybrid electrode surface
    Yao, H; Zheng, G; Hsu, P-C; Kong, D; Cha, JJ; Li, W; Seh, ZW; McDowell, MT; Yan, K; Liang, Z; Narasimhan, VK; Cui, Y
  • Using “tender” X-ray ambient pressure X-ray photoelectron spectroscopy as a direct probe of solid-liquid Interface
    Axnanda, S; Crumlin, EJ; Mao, B; Rani, S; Chang, R; Karlsson, PG; Edwards, MOM; Lundqvist, M; Moberg, R; Ross, P; Hussain, Z; Liu, Z
  • Comprehensive X-ray photoelectron spectroscopy study of the conversion reaction mechanism of CuO in lithiated thin film electrodes
    Martin, L; Martinez, H; Poinot, D; Pecquenard, B; Cras, F
  • Quantitative ToF-SIMS depth profiling of a multi-phased III–V semiconductor matrix via the analysis of secondary cluster ions
    Gong, B; Marjo, CE
  • Single-cell analysis: visualizing pharmaceutical and metabolite uptake in cells with label-free 3D mass spectrometry imaging
    Passarelli, MK; Newman, CF; Marshall, PS; West, A; Gilmore, IS; Bunch, J; Alexander, MR; Dollery, CT
  • Recent advances in real-time and in situ analysis of an electrode–electrolyte interface by mass spectrometry
    Lu, J; Hua, X; Long, Y-T
  • Organic depth profiling of a nanostructured delta layer reference material using large argon cluster ions
    Lee, J; Ninomiya, S; Matsuo, J; Gilmore, I; Seah, M; Shard, A
  • Enhancing secondary ion yields in time of flight-secondary ion mass spectrometry using water cluster primary beams
    née Rabbani, SS; Barber, A; Fletcher, JS; Lockyer, NP; Vickerman, JC
  • Semiempirical rules to determine drug sensitivity and ionization efficiency in secondary ion mass spectrometry using a model tissue sample
    Vorng, J-L; Kotowska, AM; Passarelli, MK; West, A; Marshall, PS; Havelund, R; Seah, MP; Dollery, CT; Rakowska, PD; Gilmore, IS
  • Salt effects on ion formation in desorption mass spectrometry: an investigation into the role of alkali chlorides on peak suppression in time-of-flight-secondary ion mass spectrometry
    Piwowar, AM; Lockyer, NP; Vickerman, JC