Investigation of the Backcross Progeny of Barley–Wheat Hybrids by the RAPD and RAMPO Methods

Investigation of the Backcross Progeny of Barley–Wheat Hybrids by the RAPD and RAMPO Methods The genomes of alloplasmic wheat lines were analyzed by PCR-based methods: random amplified polymorphic DNA (RAPD) and random amplified microsatellite polymorphism (RAMPO). Lines L-16(1) and L-17(2) were obtained by three backcrosses and line L-79(10), by four backcrosses of the barley–wheat hybrid Hordeum vulgare (2n = 14) (variety Nepolegayushchii) × Triticum aestivum (2n = 42) (variety Saratovskaya 29) with different common wheat varieties. These lines proved to be euploid (2n= 42). The aneuploid line L-9 (2n = 43 +t) was obtained after a second backcross of the hybridH. geniculatum All. (2n = 28) × T. aestivum (2n = 42) (Pyrotrix 28) with the variety Pyrotrix 28. The RAPD patterns of L-16(1) and L-17(1) contained fragments present only in the patterns of the parental wheat varieties and, in addition, fragments absent from the latter. This fragment from the pattern of L-16(1) was cloned. Analysis of its primary structure showed that the difference between L-16(1) and the parental wheat genotypes may be related to a mutation that had occurred during the development of the alloplasmic line at the binding site of an arbitrary primer. The genomes of plants of the lines L-79(10) and L-9 contain, in addition to the RAPD fragments of wheat, those characteristic of barley. RAMPO revealed higher polymorphism level among wheat varieties than that detected by RAPD. The hybridization patterns of the lines L-16(1), L-17(1), and L-79(10) contained fragments specific for wheat, and the patterns of L-9 contained both wheat and barley fragments. Russian Journal of Genetics Springer Journals

Investigation of the Backcross Progeny of Barley–Wheat Hybrids by the RAPD and RAMPO Methods

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Kluwer Academic Publishers-Plenum Publishers
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Biomedicine; Human Genetics
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