Investigation of Ternary Complexes: DNA–Phosphatidylcholine Liposomes–Mg2+ by Freeze-Fracture Method and Their Role in the Formation of Some Cell Structures

Investigation of Ternary Complexes: DNA–Phosphatidylcholine Liposomes–Mg2+ by Freeze-Fracture... Long-term investigations of ternary complexes: DNA–zwitterionic liposomes–divalent metal cations have revealed many details of their structure; but some questions need additional study. The conditions under which fusion or aggregation of liposomes occurs during such complex formation remain obscure. The DNA structure in the ternary complex is still unclear. In this work, using a freeze-fracture method, author demonstrate the thin structure of a complex (early attempts to observe this structure employing other electron microscopic methods, in particular cryo-TEM, have not met with success). After treatment of ternary complexes with nuclease S1, which is able to digest single-stranded DNA, local DNA unwinding in such complexes was confirmed. Author describe how the curvature of liposomes as the main factor may determine the interaction between liposomes and DNA, especially aggregation or fusion of liposomes during ternary complex formation. Therefore, interaction between lipids of membrane vesicles in cell and chromatin DNA can be the first stage of a nuclear envelope and pore complex assembly. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Investigation of Ternary Complexes: DNA–Phosphatidylcholine Liposomes–Mg2+ by Freeze-Fracture Method and Their Role in the Formation of Some Cell Structures

Loading next page...
 
/lp/springer_journal/investigation-of-ternary-complexes-dna-phosphatidylcholine-liposomes-aLc5dEks8g
Publisher
Springer-Verlag
Copyright
Copyright © 2009 by Springer Science+Business Media, LLC
Subject
Life Sciences; Human Physiology ; Biochemistry, general
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-009-9201-5
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial