Investigation of simultaneous lutein and lipid extraction from wet microalgae using Nile Red as solvatochromic shift probe

Investigation of simultaneous lutein and lipid extraction from wet microalgae using Nile Red as... Microalgae have been proposed as an alternative lutein source due to their high productivity, reliability, and versatility. In this study lutein and lipid extraction from wet Chlorella vulgaris UTEX 265 was investigated. The lutein production was monitored throughout the microalgal growth phase and several extraction parameters such as the sample size, drying method, and cell disruption method were investigated. The performance of solvents on lutein extraction was compared using Nile Red as a solvatochromic polarity probe. The simultaneous lutein and lipid extraction was also studied for different polarities using an ethanol-hexane binary solvent at the optimal solvent compositions suitable for lutein extraction. Among the solvents investigated, 3:1 (v/v) ethanol/hexane was recognized as the optimal solvent for lutein and lipid co-extraction, which contributed to a 13.03 mg g−1 lutein and 101.8 mg g−1 FAME yield. The saponifiable lipids content (86.9% w/w) was higher than conventional extraction methods. Based on our results, wet extraction approach exhibits good potential, while the bead-beater is the most suitable technique for cell disruption and lutein extraction. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Phycology Springer Journals

Investigation of simultaneous lutein and lipid extraction from wet microalgae using Nile Red as solvatochromic shift probe

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Publisher
Springer Netherlands
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Life Sciences; Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology
ISSN
0921-8971
eISSN
1573-5176
D.O.I.
10.1007/s10811-018-1405-6
Publisher site
See Article on Publisher Site

Abstract

Microalgae have been proposed as an alternative lutein source due to their high productivity, reliability, and versatility. In this study lutein and lipid extraction from wet Chlorella vulgaris UTEX 265 was investigated. The lutein production was monitored throughout the microalgal growth phase and several extraction parameters such as the sample size, drying method, and cell disruption method were investigated. The performance of solvents on lutein extraction was compared using Nile Red as a solvatochromic polarity probe. The simultaneous lutein and lipid extraction was also studied for different polarities using an ethanol-hexane binary solvent at the optimal solvent compositions suitable for lutein extraction. Among the solvents investigated, 3:1 (v/v) ethanol/hexane was recognized as the optimal solvent for lutein and lipid co-extraction, which contributed to a 13.03 mg g−1 lutein and 101.8 mg g−1 FAME yield. The saponifiable lipids content (86.9% w/w) was higher than conventional extraction methods. Based on our results, wet extraction approach exhibits good potential, while the bead-beater is the most suitable technique for cell disruption and lutein extraction.

Journal

Journal of Applied PhycologySpringer Journals

Published: Mar 6, 2018

References

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