ISSN 10214437, Russian Journal of Plant Physiology, 2015, Vol. 62, No. 3, pp. 367–374. © Pleiades Publishing, Ltd., 2015.
Original Russian Text © P.A. Poluboyarinov, N.A. Golubkina, 2015, published in Fiziologiya Rastenii, 2015, Vol. 62, No. 3, pp. 396–403.
At present, both biochemistry and pharmacology
of seleniumorganic compounds is a subject of active
studies. As established, these compounds appeared to
possess antitumor, antiviral, antimicrobial, antioxi
dant, and antitoxic activities . The investigations
related to creation of novel seleniumorganic prepara
tions displaying both medical and preventive action
under selenium deficit conditions are conducting.
However, up to date there is not any universal theory
explaining both the biochemical function of selenium
and metabolism of various selenium preparations
including organic ones.
The available literature data indicate the existence of
endogenous selenium level regulation or homeostasis in
plants . Thus, like animals, in plants selenium accu
mulation is inherent in reproductive organs  and also
rapidly developing tissues . This process was found to
take place largely in exocarp of fruits, pericarp of grain
seed envelope, periderm of tubers, protoderm of bulbs.
There is suggestion that “border selenium accumula
tion phenomenon” is explained by that selenium per
forms here defense antioxidant role .
It is known that in plants selenium is present in
the form of both nonorganic (selenates, selenites)
and organic (amino acids, proteins) compounds.
Plants are capable of assimilating and transforming
different forms of selenium, the nonorganic ones
into the organic ones and conversely [5, 6]. It was
found that in plant selenium accumulators an inten
sive formation of methylated forms of this element
was observed [7, 8]. Preferred accumulation of sele
nium in the roots as compared to that in shoots and
Investigation of Biochemical Function of Selenium
and Its Influence on the Content of Protein Fractions and Peroxidase
Activity in Maize Seedlings
P. A. Poluboyarinov
and N. A. Golubkina
FGBOU VPO “Penza State University of Architecture and Construction”, Penza;
AllRussian Research Institute of Breeding and Vegetable Crops, Moscow
Received October 6, 2014
—The release of elemental selenium on the root surface of maize (
L., variety Krasnodar
skii 29/AMV) seedlings observed in response to the addition of seleniumorganic preparation DAFS25
(diacetophenonyl selenide) to the nutrient Knop solution and accompanied by inhibition of growth of the
roots was investigated. This process was completely suppressed after the addition of cysteine to the above solu
tion containing 10
g/L DAFS25, with the inhibiting effect was attenuated as well. In addition, DAFS25
exerted impact on nitrogen exchange in the plants as judged by elevating the protein fraction of albumins in
the roots almost by a factor of two due to at the expense of decreasing the content of glutelin, prolamine, and
globulin fractions by 43, 25, and 26%, respectively. In the case of the action of DAFS25 in combination with
cysteine the quantitative composition of the above proteins very closely resembled that of the control plants
indicating that cysteine behaves as antidote. It was found that DAFS25 exerted a similar action on the con
tent of albumins in the aboveground part of maize seedlings where this value drastically increased (by a factor
of 4.5) on the background of total protein content elevation. In addition, this preparation at the highest con
centration tested stimulated the activity of peroxidase in both the roots (by 63%) and the aboveground part
of seedlings (by 112%). However, this effect of DAFS25 was strongly inhibited after addition of cysteine to
the nutrient solution. Evaluation of selenium content in the same parts of the control seedlings showed that
they contain only a little amount of this element. The highest content of selenium in the above plant tissues
was found after addition of DAFS25 to the nutrient solution that provides evidence for its decomposition
directly on the surface of the roots. Such an action of DAFS25, like its impact on the peroxidase activity, was
markedly attenuated in the presence of cysteine.
Keywords: Zea mays
, seedlings, roots, selenium metabolism, cysteine, diacetophenonyl selenide, sulfhydryl
groups, acetophenone, albumins, peroxidase
1,5 (diacetofenonyl selenide).