ISSN 10227954, Russian Journal of Genetics, 2010, Vol. 46, No. 10, pp. 1157–1159. © Pleiades Publishing, Inc., 2010.
Original Russian Text © A.M. Rusakova, G.N. Artemov, N.K. Potapova, V.N. Stegniy, 2010, published in Genetika, 2010, Vol. 46, No. 10, pp. 1311–1313.
Numerous cytogenetic studies of the order
showed that polymorphism in paracentric inversions is
common in this systematic group [1–3]. Clinal distri
bution of inversions in the areal and its seasonal cyclic
variation in polymorphic species demonstrate the adap
tive role of inversion polymorphism in nature [4–8].
Malarial mosquitoes of the
complex is a suitable model for cytogenetic studies.
The complex includes several species.
Fall inhabits nearly the shole boreal region of
Palearctic, is polymorphic, and has five inversions in
homo and heterozygous state, which are widespread
in the areal [9, 10]. The frequencies of inversions in the
significantly correlate with
climatic and ecological conditions of the mosquito
habitats and show clinal variation. This suggests an
important role of inversions in the species system of
genetic adaptation .
The data have been obtained on changes in the spe
cies areal and the karyotypic structure frequency in
populations of mosquitoes of
plex [11, 12]. The authors related these changes to the
climate warming. In view of this, it seems important to
conduct further population genetic studies of the
The aim of this study is comparative cytogenetic
analysis of inversion polymorphism in malarial mos
quitoes from several geographically isolated natural
populations of the eastern part of the species areal.
This cytogenetic study describes for the first time
natural populations of malarial mosquito
from Yakutia. Imagoes of malarial mosquitoes
from populations of towns of Sangar (1), Sirdik (2),
Kul’dyamtsi (3), and Tulagino (4) were used. The
numbers of the populations used in the data descrip
tion are given in brackets. The samples were collected
from July 17 through 19. The sample sizes were 42 (1),
63 (2), 59 (3), and 59 (4).
Polytene chromosomes of ovarian nurse cells were
prepared according to standard acetolactoorcein pro
tocol . Cytogenetic diagnostics was conducted
using standard cards of polytene chromosomes [6, 10].
The significance of the differences was evaluated
test and the MannWhitney test
implemented in the Statistica software.
Analysis of the cytogenetic data yielded the follow
ing results. All the populations possessed only the
variant with 100% frequency (figure). In
respect to chromosome 2R, the
variant was pre
dominant in each population. However, in population 1,
which is located to the north, the highest frequency of
(35%) and the sequence
(10%) was found. This was not observed in the other
three populations (figure). These results support ear
lier data [9, 10], which demonstrated that in the
northern part of the areal (Syktyvkar) the dominant
chromosome l sequence was
while at the southern
part of the areal it is absent. Homozygotes
right arm of chromosome 3 were predominant in each
population: with 100% frequency in population 1 and
with 90% frequency in populations 2–4 (figure).
Chromosome 3L is the most variable in frequency dis
Inversion Polymorphism in Malarial Mosquito
Natural Populations from the NorthEastern Region of the Range
A. M. Rusakova
, G. N. Artemov
, N. K. Potapova
, and V. N. Stegniy
Research Institute of Biology and Biophysics, Tomsk State University, Tomsk, 634050 Russia
Department of Cytology and Genetics, Tomsk State University, Tomsk, 634050 Russia
Research Institute of Biological Aspects of Cryolithozone, Yakutsk, 667980 Russia
email: firstname.lastname@example.org; email@example.com
Received January 28, 2010
—A cytogenetic study of eight natural populations of
from the northeastern part
of the species areal was conducted. Complete predominance of homozygotes XL
in the northern
populations was observed. Change in the chromosome 2 inversion frequencies from south northwards was
shown. The 2R
variant, which was not observed in the southern region, was found in the northern popula
tions. These results indicate the maintenance of chromosome frequency of the distribution of inversions XL
(in longitude) and 2R
(in latitude). The inversion frequency distribution in the examined part of the
areal have been preserved for a long time.