Plant Mol Biol (2016) 92:337–346 DOI 10.1007/s1103-016-0516-11 on sequences that stimulate gene expr Intr ession in Arabidopsis 1 1 1 1,2 Alan B. Rose · Amanda Carter · Ian Korf · Noah Kojima Received: 4 March 2016 / Accepted: 14 July 2016 / Published online: 5 August 2016 © Springer Science Business Media Dordrecht 2016 Abstract matches the NGA core of the longer motif, combining TY Key message Related motifs strongly increase gene the motifs to make G reduced the stimulating TTTTTCGA ession when added to an intr expr on located in coding ability of the TYTG motif. TTNGA Additional substitutions sequences. were used to test the contribution to IME of other residues Abstract Many introns greatly increase gene expression in the TTNGATYTG motif. The verification that these through a mechanism that remains elusive. An obstacle to motifs are active in IME will improve our ability to predict understanding intron-mediated enhancement (IME) has the stimulating ability of introns, to engineer any intron to been the difficulty of locating the specific intron sequences increase expression to a desired level, and to explore the responsible for boosting expression because they are redun - mechanism of IME by seeking factors that might
Plant Molecular Biology – Springer Journals
Published: Aug 5, 2016
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