Intron sequences that stimulate gene expression in Arabidopsis

Intron sequences that stimulate gene expression in Arabidopsis Plant Mol Biol (2016) 92:337–346 DOI 10.1007/s1103-016-0516-11 on sequences that stimulate gene expr Intr ession in Arabidopsis 1 1 1 1,2 Alan B. Rose · Amanda Carter · Ian Korf · Noah Kojima Received: 4 March 2016 / Accepted: 14 July 2016 / Published online: 5 August 2016 © Springer Science Business Media Dordrecht 2016 Abstract matches the NGA core of the longer motif, combining TY Key message Related motifs strongly increase gene the motifs to make G reduced the stimulating TTTTTCGA ession when added to an intr expr on located in coding ability of the TYTG motif. TTNGA Additional substitutions sequences. were used to test the contribution to IME of other residues Abstract Many introns greatly increase gene expression in the TTNGATYTG motif. The verification that these through a mechanism that remains elusive. An obstacle to motifs are active in IME will improve our ability to predict understanding intron-mediated enhancement (IME) has the stimulating ability of introns, to engineer any intron to been the difficulty of locating the specific intron sequences increase expression to a desired level, and to explore the responsible for boosting expression because they are redun - mechanism of IME by seeking factors that might http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Intron sequences that stimulate gene expression in Arabidopsis

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Publisher
Springer Netherlands
Copyright
Copyright © 2016 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-016-0516-1
Publisher site
See Article on Publisher Site

Abstract

Plant Mol Biol (2016) 92:337–346 DOI 10.1007/s1103-016-0516-11 on sequences that stimulate gene expr Intr ession in Arabidopsis 1 1 1 1,2 Alan B. Rose · Amanda Carter · Ian Korf · Noah Kojima Received: 4 March 2016 / Accepted: 14 July 2016 / Published online: 5 August 2016 © Springer Science Business Media Dordrecht 2016 Abstract matches the NGA core of the longer motif, combining TY Key message Related motifs strongly increase gene the motifs to make G reduced the stimulating TTTTTCGA ession when added to an intr expr on located in coding ability of the TYTG motif. TTNGA Additional substitutions sequences. were used to test the contribution to IME of other residues Abstract Many introns greatly increase gene expression in the TTNGATYTG motif. The verification that these through a mechanism that remains elusive. An obstacle to motifs are active in IME will improve our ability to predict understanding intron-mediated enhancement (IME) has the stimulating ability of introns, to engineer any intron to been the difficulty of locating the specific intron sequences increase expression to a desired level, and to explore the responsible for boosting expression because they are redun - mechanism of IME by seeking factors that might

Journal

Plant Molecular BiologySpringer Journals

Published: Aug 5, 2016

References

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