We have established a new type of genetic marker in Pellia liverworts that can be used to differentiate between closely related species within this genus. The Pellia intergenic sequences spanning the tandemly repeated nuclear tRNALeu genes were amplified using the PCR technique and, in the case of Pellia neesiana, the tRNALeu tandem region was cloned and sequenced. The length comparison of the intergenic regions allowed for the identification of a number of Pellia species. Additional experiments suggest that this marker can be used to differentiate other species of liverworts and mosses.
Plant Molecular Biology – Springer Journals
Published: Oct 6, 2004
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