Interactions of [NSI +] prion-like determinant with SUP35 and VTS1 genes in Saccharomyces cerevisiae

Interactions of [NSI +] prion-like determinant with SUP35 and VTS1 genes in Saccharomyces cerevisiae Previously we characterized [NSI +], determinant, that possesses the features of a yeast prion. This determinant causes the nonsense suppression in strains that bear different N-substituted variants of Sup35p, which is a translation release factor eRF3. As a result of the genomic screen, we identified VTS1, the overexpression of which is a phenotypic copy of [NSI +]. Here, we analyzed the influence of SUP35 and VTS1 on [NSI +]. We demonstrated nonsense suppression in the [NSI +] strains, which appears when SUP35 expression was decreased or against a background of general defects in the fidelity of translation termination. [NSI +] has also been shown to increase VTS1 mRNA amounts. These findings facilitate the insight into the mechanisms of nonsense suppression in the [NSI +] strains and narrow the range of candidates for [NSI +] determinant. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Interactions of [NSI +] prion-like determinant with SUP35 and VTS1 genes in Saccharomyces cerevisiae

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Publisher
Springer Journals
Copyright
Copyright © 2013 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795413100074
Publisher site
See Article on Publisher Site

Abstract

Previously we characterized [NSI +], determinant, that possesses the features of a yeast prion. This determinant causes the nonsense suppression in strains that bear different N-substituted variants of Sup35p, which is a translation release factor eRF3. As a result of the genomic screen, we identified VTS1, the overexpression of which is a phenotypic copy of [NSI +]. Here, we analyzed the influence of SUP35 and VTS1 on [NSI +]. We demonstrated nonsense suppression in the [NSI +] strains, which appears when SUP35 expression was decreased or against a background of general defects in the fidelity of translation termination. [NSI +] has also been shown to increase VTS1 mRNA amounts. These findings facilitate the insight into the mechanisms of nonsense suppression in the [NSI +] strains and narrow the range of candidates for [NSI +] determinant.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 10, 2013

References

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