Interaction of proteins with ionic liquid, alcohol and DMSO and in situ generation of gold nano-clusters in a cell

Interaction of proteins with ionic liquid, alcohol and DMSO and in situ generation of gold... In this review, we give a brief overview on how the interaction of proteins with ionic liquids, alcohols and dimethyl sulfoxide (DMSO) influences the stability, conformational dynamics and function of proteins/enzymes. We present experimental results obtained from fluorescence correlation spectroscopy on the effect of ionic liquid or alcohol or DMSO on the size (more precisely, the diffusion constant) and conformational dynamics of lysozyme, cytochrome c and human serum albumin in aqueous solution. The interaction of ionic liquid with biomolecules (e.g. protein, DNA etc.) has emerged as a current frontier. We demonstrate that ionic liquids are excellent stabilizers of protein and DNA and, in some cases, cause refolding of a protein already denatured by chemical denaturing agents. We show that in ethanol–water binary mixture, proteins undergo non-monotonic changes in size and dynamics with increasing ethanol content. We also discuss the effect of water–DMSO mixture on the stability of proteins. We demonstrate how large-scale molecular dynamics simulations have revealed the molecular origin of this observed phenomenon and provide a microscopic picture of the immediate environment of the biomolecules. Finally, we describe how favorable interactions of ionic liquids may be utilized for in situ generation of fluorescent gold nano-clusters for imaging a live cell. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biophysical Reviews Springer Journals

Interaction of proteins with ionic liquid, alcohol and DMSO and in situ generation of gold nano-clusters in a cell

Download PDF
12 pages
Read Article

Loading next page...
 
/lp/springer_journal/interaction-of-proteins-with-ionic-liquid-alcohol-and-dmso-and-in-situ-ukETW2PI3l
Publisher
Springer Journals
Copyright
Copyright © 2017 by International Union for Pure and Applied Biophysics (IUPAB) and Springer-Verlag GmbH Germany
Subject
Life Sciences; Biochemistry, general; Biological and Medical Physics, Biophysics; Cell Biology; Membrane Biology; Biological Techniques; Nanotechnology
ISSN
1867-2450
eISSN
1867-2469
DOI
10.1007/s12551-017-0331-1
Publisher site
See Article on Publisher Site

Abstract

In this review, we give a brief overview on how the interaction of proteins with ionic liquids, alcohols and dimethyl sulfoxide (DMSO) influences the stability, conformational dynamics and function of proteins/enzymes. We present experimental results obtained from fluorescence correlation spectroscopy on the effect of ionic liquid or alcohol or DMSO on the size (more precisely, the diffusion constant) and conformational dynamics of lysozyme, cytochrome c and human serum albumin in aqueous solution. The interaction of ionic liquid with biomolecules (e.g. protein, DNA etc.) has emerged as a current frontier. We demonstrate that ionic liquids are excellent stabilizers of protein and DNA and, in some cases, cause refolding of a protein already denatured by chemical denaturing agents. We show that in ethanol–water binary mixture, proteins undergo non-monotonic changes in size and dynamics with increasing ethanol content. We also discuss the effect of water–DMSO mixture on the stability of proteins. We demonstrate how large-scale molecular dynamics simulations have revealed the molecular origin of this observed phenomenon and provide a microscopic picture of the immediate environment of the biomolecules. Finally, we describe how favorable interactions of ionic liquids may be utilized for in situ generation of fluorescent gold nano-clusters for imaging a live cell.

Journal

Biophysical Reviews – Springer Journals

Published: Nov 16, 2017

References

  • Effect of alcohol on the structure of cytochrome C: FCS and molecular dynamics simulations
    Amin, MA; Halder, R; Ghosh, C
  • Direct binding of ethanol to bovine serum albumin: a fluorescent and 13C NMR Multiplet relaxation study
    Avdulov, NA; Chochina, SV; Daragan, VA
  • Fluorescence studies of protein thermostability in ionic liquids
    Baker, SN; McCleskey, TM; Pandey, S; Baker, GA
  • Enhanced pair Hydrophobicity in the water−dimethylsulfoxide (DMSO) binary mixture at low DMSO concentrations
    Banerjee, S; Roy, S; Bagchi, B
  • Structural transformations, composition anomalies and a dramatic collapse of linear polymer chains in dilute ethanol–water mixtures
    Banerjee, S; Ghosh, R; Bagchi, B
  • Room temperature ionic liquids meet biomolecules: a microscopic view of structure and dynamics
    Benedetto, A; Ballone, P
  • Nanoscale organization in room temperature ionic liquids: a coarse grained molecular dynamics simulation study
    Bhargava, BL; Devane, R; Klein, ML; Balasubramanian, S
  • Effects of organic solvents on protein structures: observation of a structured helical core in hen egg-white lysozyme in aqueous dimethylsulfoxide
    Bhattacharjya, S; Balaram, P
  • Groove binding mechanism of ionic liquids: a key factor in long-term stability of DNA in hydrated ionic liquids?
    Chandran, A; Ghoshdastidar, D; Senapati, S
  • Fluorescent gold nano-cluster inside a live breast cell: etching and higher uptake in cancer cell
    Chattoraj, S; Bhattacharyya, K
  • Effect of ethanol-water mixture on the structure and dynamics of lysozyme: a fluorescence correlation spectroscopy study
    Chattoraj, S; Mandal, AK; Bhattacharyya, K
  • Cancer cell imaging by in situ generated gold Nano-clusters
    Chattoraj, S; Amin, MA; Mohapatra, S; Ghosh, S; Bhattacharyya, K
  • Effect of ionic liquid on the native and denatured state of a protein covalently attached to a probe: solvation dynamics study
    Chowdhury, R; Mojumdar, SS; Chattoraj, S; Bhattacharyya, K
  • FRET between a donor and an acceptor covalently bound to human serum albumin in native and non-native states
    Chowdhury, R; Chattoraj, S; Mojumdar, SS; Bhattacharyya, K
  • Effect of alcohols on protein hydration: crystallographic analysis of hen egg-white lysozyme in the presence of alcohols
    Deshpande, A; Nimsadkar, S; Mande, SC
  • Binding characteristics and molecular mechanism of interaction between ionic liquid and DNA
    Ding, Y; Zhang, L; Xie, J; Guo, R
  • Silver nanostructures for fluorescence correlation spectroscopy: reduced volumes and increased signal intensities
    Dutta Choudhury, S; Ray, K; Lakowicz, JR
  • Biological activity of ionic liquids and their application in pharmaceutics and medicine
    Egorova, KS; Gordeev, EG; Ananikov, VP
  • Solubility and stability of cytochrome c in hydrated ionic liquids: effect of Oxo acid residues and Kosmotropicity
    Fujita, K; MacFarlane, DR; Forsyth, M
  • Versatile application of indirect Fourier transformation to structure factor analysis: from X-ray diffraction of molecular liquids to small angle scattering of protein solutions
    Fukasawa, T; Sato, T
  • Anomalous behavior of linear hydrocarbon chains in water–DMSO binary mixture at low DMSO concentration
    Ghosh, R; Banerjee, S; Chakrabarty, S; Bagchi, B
  • Solvent sensitivity of protein unfolding: dynamical study of chicken villin headpiece subdomain in water–ethanol binary mixture
    Ghosh, R; Roy, S; Bagchi, B
  • Structure and dynamics of lysozyme in DMSO–water binary mixture: fluorescence correlation spectroscopy
    Ghosh, S; Chattoraj, S; Chowdhury, R; Bhattacharyya, K
  • Ionic liquid induced dehydration and domain closure in lysozyme: FCS and MD simulation
    Ghosh, S; Parui, S; Jana, B; Bhattacharyya, K
  • Composition dependent multiple structural transformations of myoglobin in aqueous ethanol solution: a combined experimental and theoretical study
    Ghosh, R; Samajdar, RN; Bhattacharyya, AJ; Bagchi, B
  • Vibrational circular dichroism spectra of lysozyme solutions: solvent effects on thermal denaturation processes
    Giugliarelli, A; Sassi, P; Paolantoni, M
  • Amyloid protofilament formation of hen egg lysozyme in highly concentrated ethanol solution
    Goda, S; Takano, K; Yamagata, Y
  • Structure, dynamics, and activity of xylanase solvated in binary mixtures of ionic liquid and water
    Jaeger, VW; Pfaendtner, J
  • Understanding ethylammonium nitrate stabilized cytochrome c—molecular dynamics and experimental approach
    Jaganathan, M; Ramakrishnan, C; Velmurugan, D; Dhathathreyan, A
  • Unexpected effects of the alteration of structure and stability of myoglobin and hemoglobin in ammonium-based ionic liquids
    Jha, I; Attri, P; Venkatesu, P
  • Hydration effects on the fibrillation process of a globular protein: the case of human serum albumin
    Juárez, J; Alatorre-Meda, M; Cambón, A
  • Improving enzymes by using them in organic solvents
    Klibanov, AM
  • Analyzing the molecular basis of enzyme stability in ethanol/water mixtures using molecular dynamics simulations
    Lousa, D; Baptista, AM; Soares, CM
  • Proteins in organic solvents
    Mattos, C; Ringe, D
  • Protein structure and dynamics in ionic liquids. Insights from molecular dynamics simulation studies
    Micaelo, NM; Soares, CM
  • Ethanol shock and lysozyme aggregation
    Nemzer, LR; Flanders, BN; Schmit, JD
  • Preferential solvation of lysozyme in water/ethanol mixtures
    Ortore, MG; Mariani, P; Carsughi, F
  • Structural transformation of bovine serum albumin induced by dimethyl sulfoxide and probed by fluorescence correlation spectroscopy technique and additional methods
    Pabbathi, A; Patra, S; Samanta, A
  • Microheterogeneity of some Imidazolium ionic liquids as revealed by fluorescence correlation spectroscopy and lifetime studies
    Patra, S; Samanta, A
  • The hen egg-white lysozyme molecule
    Phillips, DC
  • Correct protein folding in glycerol
    Rariy, RV; Klibanov, AM
  • Chemical unfolding of chicken villin headpiece in aqueous Dimethyl Sulfoxide solution: Cosolvent concentration dependence, pathway, and microscopic mechanism
    Roy, S; Bagchi, B
  • Theoretical and computational analysis of static and dynamic anomalies in water−DMSO binary mixture at low DMSO concentrations
    Roy, S; Banerjee, S; Biyani, N; Jana, B; Bagchi, B
  • Dimethyl sulfoxide induced structural transformations and non-monotonic concentration dependence of conformational fluctuation around active site of lysozyme
    Roy, S; Jana, B; Bagchi, B
  • Emerging evidences of mesoscopic-scale complexity in neat ionic liquids and their mixtures
    Russina, O; Celso, FL; Plechkova, NV; Triolo, A
  • Water modulates the ultraslow dynamics of hydrated ionic liquids near CG rich DNA: consequences for DNA stability
    Saha, D; Kulkarni, M; Mukherjee, A
  • Effect of ethanol on folding of hen egg-white lysozyme under acidic condition
    Sasahara, K; Nitta, K
  • Diffusion of organic dyes in ionic liquid and giant micron sized ionic liquid mixed micelle: fluorescence correlation spectroscopy
    Sasmal, DK; Mandal, AK; Mondal, T; Bhattacharyya, K
  • An FCS study of unfolding and refolding of CPM-labeled human serum albumin: role of ionic liquid
    Sasmal, DK; Mondal, T; Sen Mojumdar, S
  • Enzyme aggregation in ionic liquids studied by dynamic light scattering and small angle neutron scattering
    Sate, D; Janssen, MHA; Stephens, G; Sheldon, RA; Seddon, KR; Lu, JR
  • Role of ionic liquid on the conformational dynamics in the native, molten globule, and unfolded states of Cytochrome C: a fluorescence correlation spectroscopy study
    Sen Mojumdar, S; Chowdhury, R; Chattoraj, S; Bhattacharyya, K
  • Size-dependent excited state behavior of glutathione-capped gold clusters and their light-harvesting capacity
    Stamplecoskie, KG; Kamat, PV
  • Denaturation and aggregation of hen egg lysozyme in aqueous ethanol solution studied by dynamic light scattering
    Tanaka, S; Oda, Y; Ataka, M
  • Unique spatial heterogeneity in ionic liquids
    Wang, Y; Voth, GA
  • Application of ionic liquids to energy storage and conversion materials and devices
    Watanabe, M; Thomas, ML; Zhang, S
  • Collapse of unfolded proteins in a mixture of denaturants
    Xia, Z; Das, P; Shaknovich, EI; Zhou, R
  • An ultrafast look at au Nanoclusters
    Yau, SH; Varnavski, O; Goodson, T
  • High quantum yield blue emission from water-soluble Au8 Nanodots
    Zheng, J; Petty, JT; Dickson, RM

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial