Insulin/FGF-binding Ciliary Membrane Glycoprotein from Tetrahymena

Insulin/FGF-binding Ciliary Membrane Glycoprotein from Tetrahymena Triton X-100 extracted ciliary membrane protein from isolated cilia, prepared from the protozoon Tetrahymena thermophila, were fractionated by affinity chromatography on columns with covalently bound fibroblast growth factor (FGF), insulin, or concanavalin A (ConA), respectively. The eluted proteins were further analyzed by electrophoresis on sodium dodecyl sulfate polyacrylamide gels, isoelectric focusing, and by immunoblotting techniques using antibodies against the FGF receptor, platetelet derived growth factor (PDGF) receptor α-subunit, and insulin receptor β-subunit. The particular antibodies were chosen because the peptides PDGF, FGF, insulin, and ConA are chemoattractants in this organism and corresponding binding (receptor) proteins could be expected to be identified. The Journal of Membrane Biology Springer Journals

Insulin/FGF-binding Ciliary Membrane Glycoprotein from Tetrahymena

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Copyright © Inc. by 2001 Springer-Verlag New York
Life Sciences; Biochemistry, general; Human Physiology
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