Insufficient accumulation of viral late mRNAs restricts the replicative cycle of human adenovirus type 37 in A549 cells

Insufficient accumulation of viral late mRNAs restricts the replicative cycle of human adenovirus... Most serotypes of human adenoviruses, except subgroup C viruses such as serotype 2 (Ad2) and Ad5, can grow only to low titers in vitro in human cell lines. To analyze the properties of the replicative cycles of poorly growing Ad serotypes, we compared Ad37 and Ad5 in human A549 cells. In comparison with Ad5, Ad37 showed a slower development of viral-induced cytopathic effects and prolonged cell viability. Furthermore, the maximum yields of progeny viruses were reduced by almost 100-fold. Although the amount of viral DNA and E1A mRNA in infected cells reached comparable levels between these two serotypes, the accumulation of viral late mRNAs and structural proteins in Ad37-infected cells was severely reduced. Overall, our study explains critical steps in the replicative cycle of Ad, which cause serotype-specific restrictions in permissive human cell lines. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Insufficient accumulation of viral late mRNAs restricts the replicative cycle of human adenovirus type 37 in A549 cells

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Publisher
Springer Vienna
Copyright
Copyright © 2009 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-009-0451-4
Publisher site
See Article on Publisher Site

Abstract

Most serotypes of human adenoviruses, except subgroup C viruses such as serotype 2 (Ad2) and Ad5, can grow only to low titers in vitro in human cell lines. To analyze the properties of the replicative cycles of poorly growing Ad serotypes, we compared Ad37 and Ad5 in human A549 cells. In comparison with Ad5, Ad37 showed a slower development of viral-induced cytopathic effects and prolonged cell viability. Furthermore, the maximum yields of progeny viruses were reduced by almost 100-fold. Although the amount of viral DNA and E1A mRNA in infected cells reached comparable levels between these two serotypes, the accumulation of viral late mRNAs and structural proteins in Ad37-infected cells was severely reduced. Overall, our study explains critical steps in the replicative cycle of Ad, which cause serotype-specific restrictions in permissive human cell lines.

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 2009

References

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