Initiator protein DnaA of Escherichia coli is a negative replication regulator of linear phage-plasmid N15

Initiator protein DnaA of Escherichia coli is a negative replication regulator of linear... Temperate bacteriophage N15 in the lysogenic state is incapable of integrating in the chromosome of Escherichia coli and represents a linear plasmid with covalently closed ends. The phage repA gene, the product of which possesses activities of primase and helicase, ensures replication of N15 DNA. The ori site of initiation of N15 replication contains binding sites for RepA and a potential site of binding the bacterial initiator protein DnaA. It was shown in our work that replication of miniplasmids based on N15 replicon as well as replication of N15 DNA during lytic growth do not depend on DnaA. Moreover, introducing mutations into the potential DnaA binding site increases the copy number of circular and linear miniplasmids that contain repA gene. These data suggest that DnaA is a negative rather than positive regulator of phage N15 replication. This is assumed to be caused by properties of interaction between RepA and DnaA during initiation of N15 replication or by transcriptional silencing of repA gene due to the binding of DnaA to the ori site located within the repA coding sequence. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Initiator protein DnaA of Escherichia coli is a negative replication regulator of linear phage-plasmid N15

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2007 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Microbial Genetics and Genomics; Animal Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795407010061
Publisher site
See Article on Publisher Site

Abstract

Temperate bacteriophage N15 in the lysogenic state is incapable of integrating in the chromosome of Escherichia coli and represents a linear plasmid with covalently closed ends. The phage repA gene, the product of which possesses activities of primase and helicase, ensures replication of N15 DNA. The ori site of initiation of N15 replication contains binding sites for RepA and a potential site of binding the bacterial initiator protein DnaA. It was shown in our work that replication of miniplasmids based on N15 replicon as well as replication of N15 DNA during lytic growth do not depend on DnaA. Moreover, introducing mutations into the potential DnaA binding site increases the copy number of circular and linear miniplasmids that contain repA gene. These data suggest that DnaA is a negative rather than positive regulator of phage N15 replication. This is assumed to be caused by properties of interaction between RepA and DnaA during initiation of N15 replication or by transcriptional silencing of repA gene due to the binding of DnaA to the ori site located within the repA coding sequence.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Jan 24, 2007

References

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