Initiation of gametophytic callus and somatic embryogenesis in Laminaria japonica (Phaeophyta) exposed to mutagenic agents

Initiation of gametophytic callus and somatic embryogenesis in Laminaria japonica (Phaeophyta)... Zoospores of Laminaria at the stage of zoospore germination fixed to glass slides were irradiated by γ-rays in doses of 50, 100, or 250 Gy; or treated with colchicine at a concentration of 4 × 10−5% for 5 days. The cultivation was conducted in vessels with seawater at a temperature of 12°N and illumination of 4000 lux for one month. Once a day, from day 22 to day 30, the temperature was reduced to 0°N for 12 h. As a result, in experimental samples gametophytes appeared that did not form gametangia; these appeared by the third day of cultivation, as plaques up to 2 cm in diameter (1–2 plaques per slide). In the same culture we found structures (1–2 per slide) consisting of strictly radially arranged rows of somatic cells attached to the slides. Later, these disks transformed into cones up to 0.5 cm in diameter. We recorded the development of a single-layered sporophyte of Laminaria arising from the center of such a cone. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Marine Biology Springer Journals

Initiation of gametophytic callus and somatic embryogenesis in Laminaria japonica (Phaeophyta) exposed to mutagenic agents

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2008 by MAIK Nauka
Subject
Life Sciences; Freshwater & Marine Ecology
ISSN
1063-0740
eISSN
1608-3377
D.O.I.
10.1134/S106307400805009X
Publisher site
See Article on Publisher Site

Abstract

Zoospores of Laminaria at the stage of zoospore germination fixed to glass slides were irradiated by γ-rays in doses of 50, 100, or 250 Gy; or treated with colchicine at a concentration of 4 × 10−5% for 5 days. The cultivation was conducted in vessels with seawater at a temperature of 12°N and illumination of 4000 lux for one month. Once a day, from day 22 to day 30, the temperature was reduced to 0°N for 12 h. As a result, in experimental samples gametophytes appeared that did not form gametangia; these appeared by the third day of cultivation, as plaques up to 2 cm in diameter (1–2 plaques per slide). In the same culture we found structures (1–2 per slide) consisting of strictly radially arranged rows of somatic cells attached to the slides. Later, these disks transformed into cones up to 0.5 cm in diameter. We recorded the development of a single-layered sporophyte of Laminaria arising from the center of such a cone.

Journal

Russian Journal of Marine BiologySpringer Journals

Published: Nov 8, 2008

References

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