1021-4437/02/4906- $27.00 © 2002
Russian Journal of Plant Physiology, Vol. 49, No. 6, 2002, pp. 789–791. Translated from Fiziologiya Rastenii, Vol. 49, No. 6, 2002, pp. 886–889.
Original Russian Text Copyright © 2002 by Yusufov, Alieva.
The comparative analysis of the responses of the
entire plant and its particular organs to stress factors
permits one to evaluate the consequences of differenti-
ation (theoretical interest) and to apply the results to
clone selection and resistance diagnostics.
Numerous reports were dedicated to the responses
of plant detached organs and tissues to salinity [1–4].
When evaluating the salt tolerance of various plants and
their particular parts, different researchers used differ-
ent indices: survival, the mortality rate, necrotization,
changes in organ and callus weights, etc. [5–9]. One of
the integral indices is growth [10, 11]. In some plants,
salinity suppressed predominantly shoot growth ;
in other plants, root growth . There is some evi-
dence for the high salt sensitivity of the regeneration
process . In particular, it was shown than even small
changes in the salt concentration in medium suppressed
cutting  and explant [1–3, 15, 16] regeneration
It is impossible to determine exactly which iso-
lated structure can be used for the appraisal of the entire
plant salt tolerance.
In this connection, we compared the survival and
capacity for the regeneration of the explants from vari-
ous kidney bean and eggplant organs, i.e., plants differ-
ing in their salt tolerance , under salinity condi-
tions. We studied callus formation and rhizogenesis,
i.e., the processes displaying a high sensitivity to even
small changes in the culturing conditions, including the
mineral composition of the medium. Keeping in mind
that a salt tolerance of only one structure is not sufﬁ-
cient for the characterization of the entire plant salt tol-
erance, we studied responses to salt stress of various
organs (leaves, cotyledons, and hypocotyls) of kidney
beans and eggplants. Such an investigation can be use-
ful for the elaboration of an
test system for salt-
MATERIALS AND METHODS
Kidney bean (
L., cv. Saks) and
L., cv. Almaz) plants
were used. The kidney bean is one of most salt-sensi-
tive crops; the eggplant is more resistant . Seeds
were germinated in boxes ﬁlled with washed sand.
Bean seedlings were used at the age of 5–15 days, and
eggplants, at the age of 7–10, 25–30, and 70–75 days.
Before the experiment, seedlings were washed with a
soap solution, tap water, and then distilled water.
Detached hypocotyls, leaves, and cotyledons were sur-
face-sterilized with 0.1% mercuric chloride for 10 min
and washed with sterile water three times (15, 10, and
5 min). The explants of hypocotyls, leaves, and cotyle-
dons that were 5–10 mm in size were placed on an MS
medium supplemented with growth regulators:
0.5 mg/l IBA and 2.5 mg/l BA. The explants were cul-
tured in penicillin ﬂasks (20 to 30 explants per treat-
ment) under similar conditions (room temperature of
C) and natural illumination (700 lx, 14 h). Salt
Initial Stages of Morphogenetic Changes in Detached Cotyledons,
Hypocotyls, and Leaves of Kidney Beans and Eggplants under
A. G. Yusufov and Z. M. Alieva
Department of Plant Physiology and Darwinism, Daghestan State University,
ul. Gadzhieva 43a, Makhachkala, 367025 Russia;
fax: (8722) 68-2326; e-mail: firstname.lastname@example.org
Received October 18, 2001
—The effects of salinity on the morphogenesis of various organs of kidney bean (
L.) and eggplant (
L.) plants grown in nosterile culture and of explants from these organs
were studied. Salt stress was produced by adding NaCl (0.5 and 1.0%) to water or the Murashige
and Skoog nutrient medium. Salt stress suppressed rhizogenesis, particularly profoundly in the salt-sensitive
beans. Callus cultures obtained from different organs differed in their salt tolerance, which was evaluated from
the callus growth. Under salinity conditions, homologous structures of the two crops differed markedly in sur-
vival and regeneration. Different organs and corresponding explants of one and the same plant differed in the
threshold sensitivity to salinity, which indicates the organ-speciﬁcity of salt resistance.
Key words: Phaseolus vulgaris - Solanum melongena - regeneration - tissue culture - salt resistance
: BA—benzyladenine; EC—explants of cotyledons;
EH—explants of hypocotyls; EL—explants of leaves; IBA—
indolebutiric acid; MS—Murashige and Skoog nutrient medium.