Inhibitory effects of Lepidium sativum polysaccharide extracts on TNF-α production in Escherichia coli-stimulated mouse

Inhibitory effects of Lepidium sativum polysaccharide extracts on TNF-α production in... The present study was designed to study the quantitative effects of extraction time, temperature and solvent to sample ratio on the yield of Lepidium sativum polysaccharides (LSP) using a Box–Behnken design. The activities of the optimized LSP extract were then tested in an in vivo experimental system of Escherichia coli (E. coli)-induced endotoxin shock. The optimal polysaccharide extraction conditions were established by the equation of regression and evaluation of the response surface contour plots: extraction time 5.2 h; temperature 95 °C and ratio of water to raw material 31.89 mL/g. Subsequently, an in vivo endotoxin shock was induced in mice with a single E. coli i.p. injection. Septic mice showed a substantial raise in the levels of tumor necrosis factor alpha (TNF-α) in plasma, whereas mice treated with LSP after E. coli injection showed considerable lower plasma levels of TNF-α (P < 0.05). These results suggest that LSP have beneficial effects when administered to mice with endotoxin shock by diminishing the pro-inflammatory response. The systemic activity of LSP indicated that the extract has a significant inhibitory effect against E. coli-induced inflammation by reducing the circulating levels of TNF-α. Further studies are warranted to explore the clinical implications of such observations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png 3 Biotech Springer Journals

Inhibitory effects of Lepidium sativum polysaccharide extracts on TNF-α production in Escherichia coli-stimulated mouse

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Chemistry; Biotechnology; Agriculture; Cancer Research; Bioinformatics; Stem Cells; Biomaterials
ISSN
2190-572X
eISSN
2190-5738
D.O.I.
10.1007/s13205-018-1309-9
Publisher site
See Article on Publisher Site

Abstract

The present study was designed to study the quantitative effects of extraction time, temperature and solvent to sample ratio on the yield of Lepidium sativum polysaccharides (LSP) using a Box–Behnken design. The activities of the optimized LSP extract were then tested in an in vivo experimental system of Escherichia coli (E. coli)-induced endotoxin shock. The optimal polysaccharide extraction conditions were established by the equation of regression and evaluation of the response surface contour plots: extraction time 5.2 h; temperature 95 °C and ratio of water to raw material 31.89 mL/g. Subsequently, an in vivo endotoxin shock was induced in mice with a single E. coli i.p. injection. Septic mice showed a substantial raise in the levels of tumor necrosis factor alpha (TNF-α) in plasma, whereas mice treated with LSP after E. coli injection showed considerable lower plasma levels of TNF-α (P < 0.05). These results suggest that LSP have beneficial effects when administered to mice with endotoxin shock by diminishing the pro-inflammatory response. The systemic activity of LSP indicated that the extract has a significant inhibitory effect against E. coli-induced inflammation by reducing the circulating levels of TNF-α. Further studies are warranted to explore the clinical implications of such observations.

Journal

3 BiotechSpringer Journals

Published: Jun 4, 2018

References

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