Inhibitory effect of doxycycline against dengue virus replication in vitro

Inhibitory effect of doxycycline against dengue virus replication in vitro Doxycycline is an antibiotic derived from tetracycline that possesses antimicrobial and anti-inflammatory activities. Antiviral activity of doxycycline against dengue virus has been reported previously; however, its anti-dengue properties need further investigation. This study was conducted to determine the potential activity of doxycycline against dengue virus replication in vitro . Doxycycline inhibited the dengue virus serine protease (DENV2 NS2B-NS3pro) with an IC 50 value of 52.3 ± 6.2 μM at 37 °C (normal human temperature) and 26.7 ± 5.3 μM at 40 °C (high fever temperature). The antiviral activity of doxycycline was first tested at different concentrations against DENV2 using a plaque-formation assay. The virus titter decreased significantly after applying doxycycline at levels lower than its 50 % cytotoxic concentration (CC 50 , 100 μM), showing concentration-dependent inhibition with a 50 % effective concentration (EC 50 ) of approximately 50 μM. Doxycycline significantly inhibited viral entry and post-infection replication of the four dengue serotypes, with serotype-specific inhibition (high activity against DENV2 and DENV4 compared to DENV1 and DENV3). Collectively, these findings underline the need for further experimental and clinical studies on doxycycline, utilizing its anti-dengue and anti-inflammatory activities to attenuate the clinical symptoms of dengue virus infection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Inhibitory effect of doxycycline against dengue virus replication in vitro

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1880-7
Publisher site
See Article on Publisher Site

Abstract

Doxycycline is an antibiotic derived from tetracycline that possesses antimicrobial and anti-inflammatory activities. Antiviral activity of doxycycline against dengue virus has been reported previously; however, its anti-dengue properties need further investigation. This study was conducted to determine the potential activity of doxycycline against dengue virus replication in vitro . Doxycycline inhibited the dengue virus serine protease (DENV2 NS2B-NS3pro) with an IC 50 value of 52.3 ± 6.2 μM at 37 °C (normal human temperature) and 26.7 ± 5.3 μM at 40 °C (high fever temperature). The antiviral activity of doxycycline was first tested at different concentrations against DENV2 using a plaque-formation assay. The virus titter decreased significantly after applying doxycycline at levels lower than its 50 % cytotoxic concentration (CC 50 , 100 μM), showing concentration-dependent inhibition with a 50 % effective concentration (EC 50 ) of approximately 50 μM. Doxycycline significantly inhibited viral entry and post-infection replication of the four dengue serotypes, with serotype-specific inhibition (high activity against DENV2 and DENV4 compared to DENV1 and DENV3). Collectively, these findings underline the need for further experimental and clinical studies on doxycycline, utilizing its anti-dengue and anti-inflammatory activities to attenuate the clinical symptoms of dengue virus infection.

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2014

References

  • West Nile virus: epidemiology and clinical features of an emerging epidemic in the United States
    Hayes, EB; Gubler, DJ
  • A study of the clinical activity of a gel combining monocaprin and doxycycline: a novel treatment for herpes labialis
    Skulason, S; Holbrook, WP; Thormar, H; Gunnarsson, GB; Kristmundsdottir, T

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