Inhibition of the Na/Bicarbonate Cotransporter NBCe1-A by diBAC Oxonol Dyes Relative to Niflumic Acid and a Stilbene

Inhibition of the Na/Bicarbonate Cotransporter NBCe1-A by diBAC Oxonol Dyes Relative to Niflumic... Na/HCO3 cotransporters (NBCs) are important regulators of intracellular pH (pH i ) in a variety of organ systems where acid-base status is critical for tissue function. To characterize the pharmacology of NBCs in more detail, we used the two-electrode voltage-clamp technique to examine the effect of previously identified inhibitors of anion exchanger 1 (AE1) on the activity of rat NBCe1-A expressed in Xenopus laevis oocytes. NBC-expressing oocytes voltage-clamped at −60 mV and exposed to a 5% CO2/33 mM HCO 3 − solution displayed NBC-mediated outward currents that were inhibited by either niflumic acid or one of the two bis-oxonol dyes diBA(3)C4 and diBA(5)C4. NBCe1-A was less sensitive to niflumic acid (apparent K i of 100 μM) than 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS, apparent K i of 36 μM) but more sensitive to the diBAC dyes (apparent K i of ∼10 μM). Based on current-voltage relationships, the diBAC dyes inhibited HCO 3 − -induced NBCe1-mediated inward currents more so than outward currents. NBCe1 sensitivity to the dyes was (1) lower in the presence of 40 μM DIDS, (2) unaffected by changes in external HCO 3 − concentration and (3) only modestly higher at an external Na+ concentration of 5, but not 15 or 33, mM. Therefore, the diBAC dyes compete with DIDS but not appreciably with Na+ or HCO 3 − for binding. The mechanism of diBAC inhibition of NBCe1 appears similar to that previously reported for AE1. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Inhibition of the Na/Bicarbonate Cotransporter NBCe1-A by diBAC Oxonol Dyes Relative to Niflumic Acid and a Stilbene

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Publisher
Springer-Verlag
Copyright
Copyright © 2007 by Springer Science+Business Media, LLC
Subject
Life Sciences; Human Physiology ; Biochemistry, general
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-007-9018-z
Publisher site
See Article on Publisher Site

Abstract

Na/HCO3 cotransporters (NBCs) are important regulators of intracellular pH (pH i ) in a variety of organ systems where acid-base status is critical for tissue function. To characterize the pharmacology of NBCs in more detail, we used the two-electrode voltage-clamp technique to examine the effect of previously identified inhibitors of anion exchanger 1 (AE1) on the activity of rat NBCe1-A expressed in Xenopus laevis oocytes. NBC-expressing oocytes voltage-clamped at −60 mV and exposed to a 5% CO2/33 mM HCO 3 − solution displayed NBC-mediated outward currents that were inhibited by either niflumic acid or one of the two bis-oxonol dyes diBA(3)C4 and diBA(5)C4. NBCe1-A was less sensitive to niflumic acid (apparent K i of 100 μM) than 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS, apparent K i of 36 μM) but more sensitive to the diBAC dyes (apparent K i of ∼10 μM). Based on current-voltage relationships, the diBAC dyes inhibited HCO 3 − -induced NBCe1-mediated inward currents more so than outward currents. NBCe1 sensitivity to the dyes was (1) lower in the presence of 40 μM DIDS, (2) unaffected by changes in external HCO 3 − concentration and (3) only modestly higher at an external Na+ concentration of 5, but not 15 or 33, mM. Therefore, the diBAC dyes compete with DIDS but not appreciably with Na+ or HCO 3 − for binding. The mechanism of diBAC inhibition of NBCe1 appears similar to that previously reported for AE1.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Jun 20, 2007

References

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