ISSN 10214437, Russian Journal of Plant Physiology, 2015, Vol. 62, No. 3, pp. 375–380. © Pleiades Publishing, Ltd., 2015.
Original Russian Text © Yu.I. Novitskii, G.V. Novitskaya, Yu.A. Serdyukov, T.K. Kocheshkova, D.R. Molokanov, M.V. Dobrovolskii, 2015, published in Fiziologiya Rastenii, 2015,
Vol. 62, No. 3, pp. 404–409.
The mechanisms underlying the influence of weak
permanent magnetic field (PMF) on biological objects
are not yet fully elucidated. The present study was
inspired by the hypothesis that PMF affects freeradi
cal reactions underlying peroxidation of unsaturated
fatty acids in membrane phospholipids, like it
occurred in liposomes from chicken egg yolk [1, 2].
The theoretical background of this hypothesis is that
the magnetic field (MF) induces singlet–triplet tran
sitions of radical pairs in membrane phospholipids,
thus affecting the rate of their recombination. It is sup
posed that lipid peroxidation reactions comprise sev
eral magnetosensitive stages affecting the lipid metab
olism. In this case, the yield of terminal products in
lipid peroxidation should depend on the magnetic
field strength and temperature. Alternative theoretical
mechanisms for biological action of magnetic field
consider indirect magnetoinduced changes in the
composition of lipids [3–5].
A magnetic orientation mechanism should be also
mentioned, which occurs in magnetotactic bacteria
containing magnetic inclusions composed of iron and
iron oxides in the form of ferromagnetic domain struc
tures [6, 7]. Magentosensitive cells of these organisms
possess a particular composition of membrane lipids
in the “magnetosome envelopes” that are enriched in
phosphatidylcholine and phosphatidylglycerol .
Electromagnetic background fields in the range of
microwave or low frequencies may also modulate
physiological or biochemical action of PMF on plants;
the modulation could be implemented by the principle
of resonance energy pumping [8, 9]. There is evidence
that both permanent and alternating magnetic fields
have an effect on spinselective reactions of ATP syn
thesis in mitochondria involving creatine kinase and
ATPase; the chemical reactivity of these enzymes is
determined by the magnetic moment of the nucleus
ion incorporated in these enzymes .
Accumulation of peroxides in tissues is among the
main factors of injury by elevated temperature, ioniz
ing and UV radiation, heavy metal ions, etc. [10–17],
as well as the magnetic field .
Kumar and Knowels  studied lipid peroxida
tion by measuring MDA content during longterm
storage of potato tubers (
Russet Burbank) at
. The MDA content decreased
from 123 to 61 nmol/g dry wt in the period from the
2nd to the 7th month of storage and increased to
298 nmol/g dry wt by the 28th month of storage.
In the work , lipid peroxidation products
(diene conjugates and Schiff bases) were examined in
chloroplast suspension isolated from 14dayold pea
Influence of Weak Permanent Magnetic Field on Lipid Peroxidation
in Radish Seedlings
Yu. I. Novitskii, G. V. Novitskaya, Yu. A. Serdyukov, T. K. Kocheshkova,
D. R. Molokanov, and M. V. Dobrovolskii
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276 Russia;
fax: 7 (495) 9778018; email: firstname.lastname@example.org
Received November 14, 2014
—The influence of weak permanent horizontal magnetic field (PMF, field strength ~400 A/m) on
lipid peroxidation was studied with 5dayold radish (
D.C.) seedlings grown
in a phytotron at
under illuminance of 1–6 klx and in darkness. Seedlings raised at geomagnetic
field (GMF) were used as the reference (control) sample. Under the action of PMF, the largest content of a
terminal lipid peroxidation product, MDA was observed in seedlings grown at illuminance of 2–3 klx. No
clear dependence of MDA accumulation on light intensity during seedling growth was observed. The action
of PMF altered the proportion between light and dark reactions of lipid peroxidation; lipid peroxidation
decreased in the magnetic field. During seed germination under the action of PMF, the content of MDA was
found to decrease: the largest decrease was observed in 5dayold seedlings at illuminance of 1 klx. The per
manent magnetic field acted as a correcting factor by affecting the MDA content.
Keywords: Raphanus sativus,
seedlings, weak permanent magnetic field, light–dark, illuminance variation,
: AMF—alternating magnetic field; PMF—perma
nent magnetic field; GMF—geomagnetic field; MF—magnetic
field; SOD—superoxide dismutase; TBA—thiobarbituric acid.