Influence of the Nature of the T-DNA Insertion Region on Transgene Expression in Arabidopsis thaliana

Influence of the Nature of the T-DNA Insertion Region on Transgene Expression in Arabidopsis... In the experiment reported here, effect of the nature of T-DNA integration region on the activity of the transgenes was studied by using a color marker gene in Arabidopsis thaliana. For this purpose, a pale homozygous ch-42 mutant was transformed with the wild-type copy of the gene (CH-42) using kanamycin resistance gene as a selectable marker. Two independent lines were identified in which CH-42 transgene was inactive. The T-DNA flanking sequences were recovered from these inactive and two active lines. These flanking sequences were used to examine copy number and DNA methylation of the T-DNA insertion site in active and inactive lines. Southern blots produced by using MspI/HpaII digested genomic DNA showed signs of methylation in both inactive lines. Furthermore, in one of the inactive line, the T-DNA flanking sequence probe hybridized to highly repetitive sequence. The results suggest some correlation between silencing of the transgene and methylation of its insertion region. Russian Journal of Genetics Springer Journals

Influence of the Nature of the T-DNA Insertion Region on Transgene Expression in Arabidopsis thaliana

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Copyright © 2005 by MAIK "Nauka/Interperiodica"
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
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