Influence of the culture medium composition on cattle oocyte maturation and embryogenesisin vitro

Influence of the culture medium composition on cattle oocyte maturation and embryogenesisin vitro We studied the capacity of the cattle oocyte for the resumption of meiosis and the achievement of metaphase II in various protein-free culture media (DMEM, TCM-199, Ham’s F-10, and Ham’s F-12) and the pattern of influence of the estrous serum on thein vitro development of fertilized cattle oocytes, with special reference to the time of its addition to the synthetic oviduct fluid containing BSA. In the first experimental series, it was shown that the highest number of oocytes (76.1%) resumed meiosis in DMEM medium. Meiosis was not resumed in Ham’s F-12. Intermediate results were obtained for TCM-199 (55.1%), which is commonly used for the maturation of cattle oocytesin vitro and for Ham’s F-10 (51.7%). The oocytes reached metaphase II in DMEM at a higher rate (45.3%) than in TCM-199 or Ham’s F-10 (29.4 and 8.6%, respectively). In the second experimental series, the estrous serum was added to the culture medium within 20 h (control) or 42 h (experiment) after the beginning of fertilization. The estrous serum did not inhibit the first cleavage division (the percentage of cleaving embryos did not differ reliably: 32.7 and 37.9%, respectively). However, a later serum addition to the culture medium (within 42 h after the beginning of fertilization) reliably increased the percentage of embryos that reached the blastocyst stage (6.5% in the control and 17.8% in the experiment) and the hatched blastocyst stage (2 and 9.2%, respectively). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

Influence of the culture medium composition on cattle oocyte maturation and embryogenesisin vitro

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Publisher
Springer Journals
Copyright
Copyright © 2000 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Developmental Biology; Animal Anatomy / Morphology / Histology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1007/BF02758814
Publisher site
See Article on Publisher Site

Abstract

We studied the capacity of the cattle oocyte for the resumption of meiosis and the achievement of metaphase II in various protein-free culture media (DMEM, TCM-199, Ham’s F-10, and Ham’s F-12) and the pattern of influence of the estrous serum on thein vitro development of fertilized cattle oocytes, with special reference to the time of its addition to the synthetic oviduct fluid containing BSA. In the first experimental series, it was shown that the highest number of oocytes (76.1%) resumed meiosis in DMEM medium. Meiosis was not resumed in Ham’s F-12. Intermediate results were obtained for TCM-199 (55.1%), which is commonly used for the maturation of cattle oocytesin vitro and for Ham’s F-10 (51.7%). The oocytes reached metaphase II in DMEM at a higher rate (45.3%) than in TCM-199 or Ham’s F-10 (29.4 and 8.6%, respectively). In the second experimental series, the estrous serum was added to the culture medium within 20 h (control) or 42 h (experiment) after the beginning of fertilization. The estrous serum did not inhibit the first cleavage division (the percentage of cleaving embryos did not differ reliably: 32.7 and 37.9%, respectively). However, a later serum addition to the culture medium (within 42 h after the beginning of fertilization) reliably increased the percentage of embryos that reached the blastocyst stage (6.5% in the control and 17.8% in the experiment) and the hatched blastocyst stage (2 and 9.2%, respectively).

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Nov 18, 2007

References

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