Glucosinolates (GS) are secondary plant metabolites comprising different subgroups with opposing effects on human health. Hairy root cultures (HRC) are potent biotechnological tools allowing the biosynthesis of special substances under defined conditions. HRC of Eruca sativa, a brassicaceaous plant, were used to test different strategies to enhance GS levels and to alter the profile. Additional sulphur supply in the nutrient medium increased especially aliphatic GS by 2.7-fold, but also enhanced indole GS by 1.8-fold. Ethephon as well as jasmonic acid as chemical elicitors enhanced only indole GS levels, whereby especially 4-methoxyindol-3-ylmethyl or 1-methoxyindol-3-ylmethyl GS accumulated. Jasmonic acid was used in combination with pulsed electric field treatment as physical elicitor. Already within 24 h, GS levels doubled in treated HRC compared to the control. For estimation of production potency, the GS levels of HRC were compared to contents of aerial and root parts of E. sativa sprouts. HRC showed a distinct GS profile compared to the parent plant with a higher content of indole GS when compared to sprout roots, but overall lower total GS levels. Furthermore, HRC released GS into the culture medium, which could be enhanced by jasmonic acid and pulsed electric field treatment. This could comprise an efficient strategy for a continuous GS production and mining without solvent extraction.
Plant Cell, Tissue and Organ Culture – Springer Journals
Published: Dec 1, 2017
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera