ISSN 1021-4437, Russian Journal of Plant Physiology, 2006, Vol. 53, No. 2, pp. 211–216. © MAIK “Nauka /Interperiodica” (Russia), 2006.
Original Russian Text © N.A. Rozhnova, G.A. Gerashchenkov, 2006, published in Fiziologiya Rastenii, 2006, Vol. 53, No. 2, pp. 235–242.
Inducing the resistance to various diseases, espe-
cially to pathogenic viruses, is one of more important
areas in modern plant physiology and plant defense. In
fact, viral diseases (viroses) decrease crop yield poten-
tial by 10–90% [1, 2]. In particular, potato viruses
affect potato seed-tuber production and result in the
degeneration of potato cultivars. In recent years, safe
technologies of plant defense are in the focus of inves-
tigations; they are based on the stimulation of plant nat-
ural defense systems by various factors displaying a
broad spectrum of protector activity, which permits
plant defense against diverse pathogens. Among such
compounds are glucans , 2,6-dichloroisonicotinic,
salicylic, and aminobutyric acids , synthetic activa-
tors, benzothiazole, probenazol ; chitin, chitozan and
its derivates [4, 5], and also arachidonic acid (AA) [4–8].
-14-eicosatetraenoic acid) is
a very important metabolite of linoleic acid and main
component of animal tissue lipids. However, AA is
rarely present in plants. Nevertheless, it was shown,
that AA plays an important role in the development of
plant immunity to fungal and bacterial infections due to
the activation of phytoalexins , sterols , and pro-
teins . However, the antiviral AA action on stress
metabolites is poorly studied. Some data were pub-
lished about changes in protein composition and the
activation of phytohemagglutenins in potato plants
[9, 10] and tobacco plants  after AA treatment.
The effects of AA on the plant hormonal system during
viral infection have not been yet studied.
It is known that the hormonal system plays a key
role in signal transduction, which provides for the devel-
opment of systemic induced resistance (SIR) [12, 13].
There is a general belief that, during SIR development,
the alterations in the hormonal metabolism take place.
In fact, qualitative and quantitative changes in the hor-
monal contents were observed, primarily in ethylene
[14, 15], cytokinins [16, 17], and ABA [14, 18] levels.
Besides SIR induction , kinetin was shown to
inhibit the replication cycle of PVX . These data
stimulate interest in studies of the phytohormone role in
the regulation of plant defense mechanism against virus
infection. However, all published data were obtained
long ago and concerned of different plant material. In
this connection, the aim of this work was to study the
alterations in the ABA, IAA, and CK concentrations
during the development of antivirus resistance after
potato plants with AA.
MATERIALS AND METHODS
The collection of potato plants
L., cv. Newskii) was maintained
by micropropagation of the central part of tube plants
(2–3 cm) with one axillary bud. Plants were grown in
Influence of Arachidonic Acid and Phytoviruses
on the Hormonal System of Potato Plants Grown
N. A. Rozhnova and G. A. Gerashchenkov
Institute of Biochemistry and Genetic, Ufa Research Center, Russian Academy of Sciences,
pr. Oktyabrya 71. Ufa, Bashkortostan, 450054 Russia;
fax: 8 (3472) 35-6100; e-mail: email@example.com
Received April 4, 2005
—Changes in the phytohormonal system during the development of antivirus resistance was investi-
gated. This resistance was induced by arachidonic acid (AA) in potato plants (
The plants in tubes were treated with AA 7 days before infection with tobacco mosaic virus or potato X
virus. These plants were used for ABA, IAA, and cytokinin quantiﬁcation. As a rule, phytoviruses increased
ABA and cytokinin levels and decreased that of IAA in both immunized and unimmunized plants. AA signiﬁ-
cantly decreased the phytovirus content in potato plants at least during 3 weeks. In general, AA immunization
acted as a limiting factor for increasing the ABA and cytokinin concentrations during virus pathogenesis in
experimental plants. However, no regularity in the changes of the IAA concentration was noted. The data
obtained showed that AA-induced changes in the hormonal system could be an important factor in the mecha-
nism of plant resistance against phytopatogenic viruses.
Key words: Solanum tuberosum - arachidonic acid - ABA - IAA - cytokinin - virus - antivirus resistance
: AA—arachidonic acid; CK—cytokinin; MS—
Murashige and Skoog nutrient medium; PVX—potato virus X;
TMV—tobacco mosaic virus; SIR—systemic induced resistance.