Influence of Amiprophosmethyl on the Root Cell Structure in the Herbicide-Sensitive and -Resistant Lines of Nicotiana plumbaginifolia

Influence of Amiprophosmethyl on the Root Cell Structure in the Herbicide-Sensitive and... Amiprophosmethyl (APM) is a herbicide acting as a microtubule antagonist. The effect of APM on root development in sensitive and resistant (apm5r) lines of Nicotiana plumbaginifolia was investigated by means of light and immunofluorescent microscopy and by transmission electron microscopy. The treatment of intact roots with APM (0.1–10 μM) led to an increase in the root diameter, termed swelling. The root cells in the elongation zone became almost spherical in the APM-sensitive (control) line but retained a cylindrical shape in the apm5r line. After APM treatment, the cell area on longitudinal root sections decreased reliably in the elongation zone but significantly increased in the meristem zone for both lines. A partial destruction of the cortical microtubule network was observed in the apm5r line, and the full degradation of microtubules was evident in the sensitive line. Analysis of the ultrastructure did not reveal any changes in the main cytoplasmic organelles, but showed disturbances of the cell-wall formation. In the meristem zone, some changes in the nucleus structure and the appearance of polynucleate cells were observed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Influence of Amiprophosmethyl on the Root Cell Structure in the Herbicide-Sensitive and -Resistant Lines of Nicotiana plumbaginifolia

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1015501304476
Publisher site
See Article on Publisher Site

Abstract

Amiprophosmethyl (APM) is a herbicide acting as a microtubule antagonist. The effect of APM on root development in sensitive and resistant (apm5r) lines of Nicotiana plumbaginifolia was investigated by means of light and immunofluorescent microscopy and by transmission electron microscopy. The treatment of intact roots with APM (0.1–10 μM) led to an increase in the root diameter, termed swelling. The root cells in the elongation zone became almost spherical in the APM-sensitive (control) line but retained a cylindrical shape in the apm5r line. After APM treatment, the cell area on longitudinal root sections decreased reliably in the elongation zone but significantly increased in the meristem zone for both lines. A partial destruction of the cortical microtubule network was observed in the apm5r line, and the full degradation of microtubules was evident in the sensitive line. Analysis of the ultrastructure did not reveal any changes in the main cytoplasmic organelles, but showed disturbances of the cell-wall formation. In the meristem zone, some changes in the nucleus structure and the appearance of polynucleate cells were observed.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 13, 2004

References

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