Infectivity of the cloned components of a begomovirus: DNA beta complex causing chilli leaf curl disease in India

Infectivity of the cloned components of a begomovirus: DNA beta complex causing chilli leaf curl... The full-length genome of a begomovirus and its cognate DNA-β satellite component associated with chilli leaf curl disease (ChLCD), originating from Varanasi, India, were cloned. Sequence analysis revealed that the viral genome (EF190217) is 2,750 bp and the DNA-β satellite (EF190215) is 1,361 bp in length. Agroinoculation with partial tandem repeats of the viral genome along with the satellite induced symptoms typical of ChLCD in chilli and Nicotiana benthamiana . However, symptom expression was delayed and milder when the viral genome was agroinoculated alone in these hosts. Sequence comparisons revealed that the genome had the highest sequence identity (95%) with that of chilli leaf curl virus-PK(PK:Mul:98). The DNA-β satellite shared maximum sequence identity (88%) with a DNA-β satellite associated with tomato leaf curl disease from Rajasthan (ToLCBDB-(IN:Raj:03)). These results demonstrate that ChLCD is caused by a complex consisting of the monopartite chilli leaf curl virus and a DNA-β satellite component. This is the first experimental demonstration of Koch’s postulates using cloned DNA molecules associated with chilli leaf curl disease. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Infectivity of the cloned components of a begomovirus: DNA beta complex causing chilli leaf curl disease in India

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Publisher
Springer Vienna
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-007-0017-2
Publisher site
See Article on Publisher Site

Abstract

The full-length genome of a begomovirus and its cognate DNA-β satellite component associated with chilli leaf curl disease (ChLCD), originating from Varanasi, India, were cloned. Sequence analysis revealed that the viral genome (EF190217) is 2,750 bp and the DNA-β satellite (EF190215) is 1,361 bp in length. Agroinoculation with partial tandem repeats of the viral genome along with the satellite induced symptoms typical of ChLCD in chilli and Nicotiana benthamiana . However, symptom expression was delayed and milder when the viral genome was agroinoculated alone in these hosts. Sequence comparisons revealed that the genome had the highest sequence identity (95%) with that of chilli leaf curl virus-PK(PK:Mul:98). The DNA-β satellite shared maximum sequence identity (88%) with a DNA-β satellite associated with tomato leaf curl disease from Rajasthan (ToLCBDB-(IN:Raj:03)). These results demonstrate that ChLCD is caused by a complex consisting of the monopartite chilli leaf curl virus and a DNA-β satellite component. This is the first experimental demonstration of Koch’s postulates using cloned DNA molecules associated with chilli leaf curl disease.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2008

References

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