Infectivity of the cloned components of a begomovirus: DNA beta complex causing chilli leaf curl disease in India

Infectivity of the cloned components of a begomovirus: DNA beta complex causing chilli leaf curl... The full-length genome of a begomovirus and its cognate DNA-β satellite component associated with chilli leaf curl disease (ChLCD), originating from Varanasi, India, were cloned. Sequence analysis revealed that the viral genome (EF190217) is 2,750 bp and the DNA-β satellite (EF190215) is 1,361 bp in length. Agroinoculation with partial tandem repeats of the viral genome along with the satellite induced symptoms typical of ChLCD in chilli and Nicotiana benthamiana . However, symptom expression was delayed and milder when the viral genome was agroinoculated alone in these hosts. Sequence comparisons revealed that the genome had the highest sequence identity (95%) with that of chilli leaf curl virus-PK(PK:Mul:98). The DNA-β satellite shared maximum sequence identity (88%) with a DNA-β satellite associated with tomato leaf curl disease from Rajasthan (ToLCBDB-(IN:Raj:03)). These results demonstrate that ChLCD is caused by a complex consisting of the monopartite chilli leaf curl virus and a DNA-β satellite component. This is the first experimental demonstration of Koch’s postulates using cloned DNA molecules associated with chilli leaf curl disease. Archives of Virology Springer Journals

Infectivity of the cloned components of a begomovirus: DNA beta complex causing chilli leaf curl disease in India

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Springer Vienna
Copyright © 2008 by Springer-Verlag
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
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