Three isolates of cucumber fruit mottle mosaic virus (CFMMV) were collected from melon, cucumber, and pumpkin plants in Korea. A full-length cDNA clone of CFMMV-Cm (melon isolate) was produced and evaluated for infectivity after T7 transcription in vitro (pT7CF-Cm flc ). The complete CFMMV genome sequence of the infectious clone pT7CF-Cm flc was determined. The genome of CFMMV-Cm consisted of 6,571 nucleotides and shared high nucleotide sequence identity (98.8 %) with the Israel isolate of CFMMV. Based on the infectious clone pT7CF-Cm flc , a CaMV 35S-promoter driven cDNA clone (p35SCF-Cm flc ) was subsequently constructed and sequenced. Mechanical inoculation with RNA transcripts of pT7CF-Cm flc and agro-inoculation with p35SCF-Cm flc resulted in systemic infection of cucumber and melon, producing symptoms similar to those produced by CFMMV-Cm. Progeny virus in infected plants was detected by RT-PCR, western blot assay, and transmission electron microscopy.
Archives of Virology – Springer Journals
Published: Jul 1, 2014
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