Infection of Rosellinia necatrix with purified viral particles of a member of Partitiviridae (RnPV1-W8)

Infection of Rosellinia necatrix with purified viral particles of a member of Partitiviridae... Isolate W8 of the white root rot fungus, Rosellinia necatrix , harbors three dsRNA segments, L1-, L2- and M-dsRNAs, and showed an irregular colony margin, slow growth, and moderate virulence. The M-dsRNA was previously shown to be the genome of a partitivirus, RnPV1-W8. Here a transfection protocol was developed for RnPV1-W8. Protoplasts of two virus-free isolates of R. necatrix were inoculated with purified viral particles using a polyethylene glycol-mediated method. Virus infection was confirmed by electrophoresis and Northern analysis. RnPV1-W8 introduced into the new host isolates was transmissible via hyphal anastomosis. However, the infection had no effect on the morphology and virulence of infected isolates of R. necatrix . This is the first report on the transfection of a partitivirus for R. necatrix . Archives of Virology Springer Journals

Infection of Rosellinia necatrix with purified viral particles of a member of Partitiviridae (RnPV1-W8)

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Copyright © 2006 by Springer-Verlag/Wien
Biomedicine; Medical Microbiology; Infectious Diseases; Virology
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