Infection of Rosellinia necatrix with purified viral particles of a member of Partitiviridae (RnPV1-W8)

Infection of Rosellinia necatrix with purified viral particles of a member of Partitiviridae... Isolate W8 of the white root rot fungus, Rosellinia necatrix , harbors three dsRNA segments, L1-, L2- and M-dsRNAs, and showed an irregular colony margin, slow growth, and moderate virulence. The M-dsRNA was previously shown to be the genome of a partitivirus, RnPV1-W8. Here a transfection protocol was developed for RnPV1-W8. Protoplasts of two virus-free isolates of R. necatrix were inoculated with purified viral particles using a polyethylene glycol-mediated method. Virus infection was confirmed by electrophoresis and Northern analysis. RnPV1-W8 introduced into the new host isolates was transmissible via hyphal anastomosis. However, the infection had no effect on the morphology and virulence of infected isolates of R. necatrix . This is the first report on the transfection of a partitivirus for R. necatrix . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Infection of Rosellinia necatrix with purified viral particles of a member of Partitiviridae (RnPV1-W8)

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Publisher
Springer-Verlag
Copyright
Copyright © 2006 by Springer-Verlag/Wien
Subject
Biomedicine; Medical Microbiology; Infectious Diseases; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-005-0662-2
Publisher site
See Article on Publisher Site

Abstract

Isolate W8 of the white root rot fungus, Rosellinia necatrix , harbors three dsRNA segments, L1-, L2- and M-dsRNAs, and showed an irregular colony margin, slow growth, and moderate virulence. The M-dsRNA was previously shown to be the genome of a partitivirus, RnPV1-W8. Here a transfection protocol was developed for RnPV1-W8. Protoplasts of two virus-free isolates of R. necatrix were inoculated with purified viral particles using a polyethylene glycol-mediated method. Virus infection was confirmed by electrophoresis and Northern analysis. RnPV1-W8 introduced into the new host isolates was transmissible via hyphal anastomosis. However, the infection had no effect on the morphology and virulence of infected isolates of R. necatrix . This is the first report on the transfection of a partitivirus for R. necatrix .

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2006

References

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