Infection of primary human placental fibroblasts with HIV-1, HIV-2, and SIV

Infection of primary human placental fibroblasts with HIV-1, HIV-2, and SIV Cells with fibroblast-like features were isolated from the villous tissue of normal term human placentas. Immunocytochemical characterization of the cells showed that they were vimentin-positive but negative for factor-VIII, CD14 and CD4. Thus, the cells are mesenchymal and are not endothelial cells, macrophages or trophoblast. These cells were exposed to nine different cell-free virus isolates, including seven isolates of human immunodeficiency virus type 1 (HIV-1), one HIV-2 isolate and one simian immunodeficiency virus isolate (SIV mac251 ). The susceptibility of the cells to infection was evaluated by immunocytochemical and virological techniques. No evidence of infection could be found using immunofluorescence microscopy or by p24 antigen capture and reverse transcriptase assays. However, virus rescue experiments using 11 different target cell types provided evidence that the placental fibroblasts were susceptible to infection with HIV-1 Lai , HIV-1 IIIB , HIV-2 CBL-20 , and SIV mac251 , yet were resistant to infection by all other isolates. The infected fibroblasts exhibited neither cytopathic effects nor released virus into the culture medium. For each infected fibroblast population, some, but not all, indicator target cell lines or human peripheral blood mononuclear cells were able to rescue the respective virus. Based on these observations, we conclude that placental fibroblasts can be infected with HIV during transplacental transmission and could act as virus reservoirs, capable of infecting other fetal cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Infection of primary human placental fibroblasts with HIV-1, HIV-2, and SIV

Loading next page...
 
/lp/springer_journal/infection-of-primary-human-placental-fibroblasts-with-hiv-1-hiv-2-and-eTpM3mzquE
Publisher
Springer-Verlag
Copyright
Copyright © Wien by 1997 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050238
Publisher site
See Article on Publisher Site

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial