Induction of a β-phaseolin promoter by exogenous abscisic acid in tobacco: developmental regulation and modulation by external sucrose and Ca2+ ions

Induction of a β-phaseolin promoter by exogenous abscisic acid in tobacco: developmental... Phaseolin genes are induced by unidentified factors at the onset of seed maturation in embryos of both Phaseolus and tobacco. We show that in tobacco, expression of a β-phaseolin promoter-GUS (PHSβ-uidA) mRNA and the corresponding GUS activity, could be induced by abscisic acid (ABA). The effect paralleled an increase in the amount of endogenous 12S globulin (Glb12S) mRNA. In contrast, ABA repressed the expression of isocitrate lyase (ll9) mRNA. The responses of PHSβ-uidA and Glb12S to ABA declined markedly between 11 and 13 DAF, indicating that they are developmentally regulated. We also show evidence that the ABA response of PHSβ-uidA can be modulated by the external concentrations of sucrose and Ca2+ ion. These compounds inhibited the response if added to the medium separately, in the concentration ranges of 80-200 mM for sucrose and 0.76–20 mM for CaCl2. However, the presence of both sucrose and CaCl2 restored the ABA response to 20–40% of the maximum value measured in sucrose- and CaCl2-free media. These results suggest that ABA induction of β-phaseolin gene expression is modulated by developmental signals and by the external supply of sucrose and calcium to the embryos. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Induction of a β-phaseolin promoter by exogenous abscisic acid in tobacco: developmental regulation and modulation by external sucrose and Ca2+ ions

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Publisher
Springer Journals
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005999725715
Publisher site
See Article on Publisher Site

Abstract

Phaseolin genes are induced by unidentified factors at the onset of seed maturation in embryos of both Phaseolus and tobacco. We show that in tobacco, expression of a β-phaseolin promoter-GUS (PHSβ-uidA) mRNA and the corresponding GUS activity, could be induced by abscisic acid (ABA). The effect paralleled an increase in the amount of endogenous 12S globulin (Glb12S) mRNA. In contrast, ABA repressed the expression of isocitrate lyase (ll9) mRNA. The responses of PHSβ-uidA and Glb12S to ABA declined markedly between 11 and 13 DAF, indicating that they are developmentally regulated. We also show evidence that the ABA response of PHSβ-uidA can be modulated by the external concentrations of sucrose and Ca2+ ion. These compounds inhibited the response if added to the medium separately, in the concentration ranges of 80-200 mM for sucrose and 0.76–20 mM for CaCl2. However, the presence of both sucrose and CaCl2 restored the ABA response to 20–40% of the maximum value measured in sucrose- and CaCl2-free media. These results suggest that ABA induction of β-phaseolin gene expression is modulated by developmental signals and by the external supply of sucrose and calcium to the embryos.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 6, 2004

References

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