Independent species status of Iris vorobievii N.S. Pavlova, Iris mandshurica Maxim., and Iris humilis Georgi (Iridaceae): Evidence from the nuclear and chloroplast genomes

Independent species status of Iris vorobievii N.S. Pavlova, Iris mandshurica Maxim., and Iris... -Nuclear (RAPD) and chloroplast (trnH-psbA, atpB-rbcL, rps4, trnL-trnF, and trnS-trnG) molecular DNA markers were used to study the populations of closely related species of the genus Iris L.: Iris vorobievii N.S. Pavlova, I. mandshurica Maxim., and I. humilis Georgi. An analysis of 243 RAPD fragments made it possible to calculate the main population parameters and to identify species-specific RAPD markers. Differences that discriminated the species were found in all but one (trnL-trnF) cpDNA region. The total length of the four variable cpDNA regions was 3640 bp, indels included. Their variation was low. A total of 22 variable sites (0.63%) were found; of these, 13 were parsimony informative and 9 were noninformative. Analysis of molecular variance (AMOVA) revealed significantly (P < 0.0001) high differentiation of both nuclear (F ST = 0.681) and chloroplast (F ST = 0.854) genomes for the species examined. Thus, a multigene approach confirmed the independent species status of I. vorobievii, I. mandshurica, and I. humilis and showed that I. vorobievii and I. mandshurica, but not I. humilis, are found in Primorye. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Independent species status of Iris vorobievii N.S. Pavlova, Iris mandshurica Maxim., and Iris humilis Georgi (Iridaceae): Evidence from the nuclear and chloroplast genomes

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Publisher
Springer Journals
Copyright
Copyright © 2009 by Pleiades Publishing, Ltd.
Subject
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795409110143
Publisher site
See Article on Publisher Site

Abstract

-Nuclear (RAPD) and chloroplast (trnH-psbA, atpB-rbcL, rps4, trnL-trnF, and trnS-trnG) molecular DNA markers were used to study the populations of closely related species of the genus Iris L.: Iris vorobievii N.S. Pavlova, I. mandshurica Maxim., and I. humilis Georgi. An analysis of 243 RAPD fragments made it possible to calculate the main population parameters and to identify species-specific RAPD markers. Differences that discriminated the species were found in all but one (trnL-trnF) cpDNA region. The total length of the four variable cpDNA regions was 3640 bp, indels included. Their variation was low. A total of 22 variable sites (0.63%) were found; of these, 13 were parsimony informative and 9 were noninformative. Analysis of molecular variance (AMOVA) revealed significantly (P < 0.0001) high differentiation of both nuclear (F ST = 0.681) and chloroplast (F ST = 0.854) genomes for the species examined. Thus, a multigene approach confirmed the independent species status of I. vorobievii, I. mandshurica, and I. humilis and showed that I. vorobievii and I. mandshurica, but not I. humilis, are found in Primorye.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Nov 12, 2009

References

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