-Nuclear (RAPD) and chloroplast (trnH-psbA, atpB-rbcL, rps4, trnL-trnF, and trnS-trnG) molecular DNA markers were used to study the populations of closely related species of the genus Iris L.: Iris vorobievii N.S. Pavlova, I. mandshurica Maxim., and I. humilis Georgi. An analysis of 243 RAPD fragments made it possible to calculate the main population parameters and to identify species-specific RAPD markers. Differences that discriminated the species were found in all but one (trnL-trnF) cpDNA region. The total length of the four variable cpDNA regions was 3640 bp, indels included. Their variation was low. A total of 22 variable sites (0.63%) were found; of these, 13 were parsimony informative and 9 were noninformative. Analysis of molecular variance (AMOVA) revealed significantly (P < 0.0001) high differentiation of both nuclear (F ST = 0.681) and chloroplast (F ST = 0.854) genomes for the species examined. Thus, a multigene approach confirmed the independent species status of I. vorobievii, I. mandshurica, and I. humilis and showed that I. vorobievii and I. mandshurica, but not I. humilis, are found in Primorye.
Russian Journal of Genetics – Springer Journals
Published: Nov 12, 2009
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera