Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomal-lysosomal system acidification

Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with... Treatment of porcine kidney (PK-15) cells with either interferon-gamma (IFN-γ) or endosomal- lysosomal system acidification inhibitors increases replication of porcine circovirus type 2 (PCV2). In the present study, the effect of a combination of these treatments on the number of infected cells and virus yield was tested. The number of PCV2 (Stoon-1010)-infected PK-15 cells increased in cells treated with ammonium chloride (445 ± 39% increase), IFN-γ (446 ± 8%), ammonium chloride + IFN-γ (1721 ± 283%), chloroquine diphosphate (1037 ± 121%), chloroquine diphosphate + IFN-γ (2199 ± 255%), monensin (950 ± 178%) and monensin + IFN-γ (1948 ± 60%). Combined IFN-γ and endosomal-lysosomal system acidification inhibitors increased PCV2 yield by up to 50 times compared to untreated PK-15. This augmented virus replication in PK-15 cells may be helpful in the production of PCV2 vaccines. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomal-lysosomal system acidification

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Publisher
Springer-Verlag
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-007-1092-0
Publisher site
See Article on Publisher Site

Abstract

Treatment of porcine kidney (PK-15) cells with either interferon-gamma (IFN-γ) or endosomal- lysosomal system acidification inhibitors increases replication of porcine circovirus type 2 (PCV2). In the present study, the effect of a combination of these treatments on the number of infected cells and virus yield was tested. The number of PCV2 (Stoon-1010)-infected PK-15 cells increased in cells treated with ammonium chloride (445 ± 39% increase), IFN-γ (446 ± 8%), ammonium chloride + IFN-γ (1721 ± 283%), chloroquine diphosphate (1037 ± 121%), chloroquine diphosphate + IFN-γ (2199 ± 255%), monensin (950 ± 178%) and monensin + IFN-γ (1948 ± 60%). Combined IFN-γ and endosomal-lysosomal system acidification inhibitors increased PCV2 yield by up to 50 times compared to untreated PK-15. This augmented virus replication in PK-15 cells may be helpful in the production of PCV2 vaccines.

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2008

References

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