J Mater Sci: Mater Med (2017) 28:150
TISSUE ENGINEERING CONSTRUCTS AND CELL SUBSTRATES
In vivo immunological properties research on mesenchymal stem
cells based engineering cartilage by a dialyzer pocket model
Received: 14 February 2017 / Accepted: 1 August 2017 / Published online: 22 August 2017
© Springer Science+Business Media, LLC 2017
Abstract As the seed cells, the immune properties of the
mesenchymal stem cells are important for the tissue engi-
neering restoring effect. But the in vivo research model is
lacking. In the study, based on a dialyzer pocket model,
changes in immunological properties and the differentiation
of seeded mesenchymal stem cells (MSCs) in collagen
hydrogel were studied in muscle and articular cavity
implantation, respectively. The results showed that collagen
hydrogel can induce MSCs to form cartilage tissue, followed
by alteration of immunological properties. In muscle
implantation, relatively low expression of major histo-
compatibility complex (MHC) molecules and low level of
one-way mixed lymphocyte reactions (MLR) on the seeded
MSCs were observed, but only a little cartilage tissue formed.
In articular cavity implantation, more cartilage tissue formed,
but higher MHC expressions and MLR level were found.
Results indicated that the immunomodulation and the
cartilage formation of the seeded MSCs will be impacted by
the scaffold and the environment of the in vivo implanted site.
The dialyzer pocket model can be used for the in vivo
research for the MSC-based strategy of the tissue engineering,
especially for the optimization of the immunomodulation.
Keywords Mesenchymal stem cells
Cartilage tissue engineering
Mesenchymal stem cells (MSCs) are one of the common
seeded cells in tissue engineering . Unlike the cells
which hold a determined direction of differentiation,
MSCs have multi-directional differentiation capability [2, 3].
* Jie Liang
* Yujiang Fan
National Engineering Research Center for Biomaterials, Sichuan
University, Chengdu, China