Features of in vitro regeneration of Fritillaria sonnikovae from bulb scales were studied. The initi-ation of shoot formation was obtained on a nutrient medium BDS, supplemented with 5 µM 6-benzylami-nopurine and 2 µM α-naphthaleneacetic acid. Optimization of propagation-stage was carried out usingobtained microbulbs as explants. High regenerative response of explants and shoot multiplication rate wereobserved on both media supplemented with growth regulators (up to 47% and 4.2 ± 0.6 shoots per explant,respectively) and hormone-free medium (48% and 4.1 ± 0.2 shoots per explant, respectively). It has beenestablished that the addition of growth regulators on the stage of cultivation does not cause increased mor-phogenic response but contributes to the accelerated initiation and development of microbulbs. Morpholog-ical and histological analysis revealed the dynamics of the formation of shoots de novo. In vitro developmentof F. sonnikovae follows the path of direct organogenesis from the epidermal tissue of the explant.
Russian Journal of Developmental Biology – Springer Journals
Published: Jul 26, 2015
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