In vitro regeneration from bulbous scales of Fritillaria sonnikovae, an endemic species

In vitro regeneration from bulbous scales of Fritillaria sonnikovae, an endemic species Features of in vitro regeneration of Fritillaria sonnikovae from bulb scales were studied. The initi-ation of shoot formation was obtained on a nutrient medium BDS, supplemented with 5 µM 6-benzylami-nopurine and 2 µM α-naphthaleneacetic acid. Optimization of propagation-stage was carried out usingobtained microbulbs as explants. High regenerative response of explants and shoot multiplication rate wereobserved on both media supplemented with growth regulators (up to 47% and 4.2 ± 0.6 shoots per explant,respectively) and hormone-free medium (48% and 4.1 ± 0.2 shoots per explant, respectively). It has beenestablished that the addition of growth regulators on the stage of cultivation does not cause increased mor-phogenic response but contributes to the accelerated initiation and development of microbulbs. Morpholog-ical and histological analysis revealed the dynamics of the formation of shoots de novo. In vitro developmentof F. sonnikovae follows the path of direct organogenesis from the epidermal tissue of the explant. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

In vitro regeneration from bulbous scales of Fritillaria sonnikovae, an endemic species

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Inc.
Subject
Life Sciences; Developmental Biology; Animal Anatomy / Morphology / Histology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360415040050
Publisher site
See Article on Publisher Site

Abstract

Features of in vitro regeneration of Fritillaria sonnikovae from bulb scales were studied. The initi-ation of shoot formation was obtained on a nutrient medium BDS, supplemented with 5 µM 6-benzylami-nopurine and 2 µM α-naphthaleneacetic acid. Optimization of propagation-stage was carried out usingobtained microbulbs as explants. High regenerative response of explants and shoot multiplication rate wereobserved on both media supplemented with growth regulators (up to 47% and 4.2 ± 0.6 shoots per explant,respectively) and hormone-free medium (48% and 4.1 ± 0.2 shoots per explant, respectively). It has beenestablished that the addition of growth regulators on the stage of cultivation does not cause increased mor-phogenic response but contributes to the accelerated initiation and development of microbulbs. Morpholog-ical and histological analysis revealed the dynamics of the formation of shoots de novo. In vitro developmentof F. sonnikovae follows the path of direct organogenesis from the epidermal tissue of the explant.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: Jul 26, 2015

References

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