A protocol was developed for propagation and in vitro conservation of a rare traditional medicinal plant, Globba marantina L. The present slow growth protocol was achieved for long term conservation of plants using both solid and liquid Murashige and Skoog media with different carbon sources and growth hormones. Half-Murashige and Skoog supplemented with Kinetin (3 mg/l) and Naphthalene acetic acid (0.5 mg/l) was found to be optimum with sucrose (10 g/l) and mannitol (10 g/l). Subculture duration could be significantly enhanced and the regenerated plants were successfully conserved for a maximum period of 220 days. Plants regenerated from conserved cultures were successfully established in soil. On the basis of 12 inter simple sequence repeat primers and morphological characteristic analysis from field up to two generations, no significant variation was observed between the control and in vitro regenerated plants. The present protocol for the first time reports mass propagation and genetic stability of G. marantina L. which could be used for commercial purposes.
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals
Published: Jul 4, 2016
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.
All for just $49/month
Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.
Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.
It’s easy to organize your research with our built-in tools.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera