In vitro morphogenesis and micropropagation of Aechmea ramosa var. ramosa Mart. ex Schult. f. (Bromeliaceae) from leaf explants

In vitro morphogenesis and micropropagation of Aechmea ramosa var. ramosa Mart. ex Schult. f.... Aechmea ramosa Mart. ex Schult. f. is an endemic bromeliad of the Brazilian Atlantic Forest. The current habitat degradation of this hotspot biome threatens this species, which besides having an important ecological role, is also of invaluable ornamental interest. Plant tissue culture has been used in mass propagation and conservation of various bromeliads. We have established a micropropagation protocol for A. ramosa var. ramosa using leaf explants grown in MS medium supplemented with 2 μM of 1-naphthaleneacetic acid (NAA) and 2 μM of 6-benzylaminopurine (BAP) that showed higher values of shoot induction. NAA and BAP are associated with the production of proteins involved in stress response modulation, metabolic activity, and cell division, the latter being involved in inducing the differentiation of competent cells. After 120 d of culture, each explant presented 28.9 shoots with an average size of 27.8 mm, with no variation in either Stomatal Index or density of the regenerated shoots. Plantlets measuring above 15-mm height were successfully acclimatized, presenting 100% survival rate. Thus, this protocol can be used for mass propagation of A. ramosa, and to supply demand for the market of ornamental plants. Furthermore, it represents an important tool for the conservation of this species and maintenance of an in vitro germplasm. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png In Vitro Cellular & Developmental Biology - Plant Springer Journals

In vitro morphogenesis and micropropagation of Aechmea ramosa var. ramosa Mart. ex Schult. f. (Bromeliaceae) from leaf explants

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Publisher
Springer Journals
Copyright
Copyright © 2018 by The Society for In Vitro Biology
Subject
Life Sciences; Plant Sciences; Cell Biology; Developmental Biology; Plant Breeding/Biotechnology; Plant Genetics and Genomics
ISSN
1054-5476
eISSN
1475-2689
D.O.I.
10.1007/s11627-018-9907-0
Publisher site
See Article on Publisher Site

Abstract

Aechmea ramosa Mart. ex Schult. f. is an endemic bromeliad of the Brazilian Atlantic Forest. The current habitat degradation of this hotspot biome threatens this species, which besides having an important ecological role, is also of invaluable ornamental interest. Plant tissue culture has been used in mass propagation and conservation of various bromeliads. We have established a micropropagation protocol for A. ramosa var. ramosa using leaf explants grown in MS medium supplemented with 2 μM of 1-naphthaleneacetic acid (NAA) and 2 μM of 6-benzylaminopurine (BAP) that showed higher values of shoot induction. NAA and BAP are associated with the production of proteins involved in stress response modulation, metabolic activity, and cell division, the latter being involved in inducing the differentiation of competent cells. After 120 d of culture, each explant presented 28.9 shoots with an average size of 27.8 mm, with no variation in either Stomatal Index or density of the regenerated shoots. Plantlets measuring above 15-mm height were successfully acclimatized, presenting 100% survival rate. Thus, this protocol can be used for mass propagation of A. ramosa, and to supply demand for the market of ornamental plants. Furthermore, it represents an important tool for the conservation of this species and maintenance of an in vitro germplasm.

Journal

In Vitro Cellular & Developmental Biology - PlantSpringer Journals

Published: May 30, 2018

References

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