In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration

In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial... Purpose The study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endome- trial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring. Methods Human endometrial stromal fibroblasts (eSF), endometrial mesenchymal stem cells (eMSC), bone marrow-derived mesenchymal stem cells (BM-MSC), and Ishikawa endometrial adenocarcinoma cells (IC) were cultured with/without 5% activated (a) PRP, non-activated (na) PRP, aPPP (platelet-poor-plasma), and naPPP. Treatment effects were evaluated with cell proliferation (WST-1), wound healing, and chemotaxis Transwell migration assays. Mesenchymal-to-epithelial transition (MET) was evaluated by cytokeratin and vimentin expression. Differential gene expression of various markers was analyzed by multiplex Q-PCR. Results Activated PRP enhanced migration of all cell types, compared to naPRP, aPPP, naPPP, and vehicle controls, in a time- dependent manner (p < 0.05). The WST-1 assay showed increased stromal and mesenchymal cell proliferation by aPRP vs. naPRP, aPPP, and naPPP (p < 0.05), while IC proliferation was enhanced by aPRP and aPPP (p < 0.05). There was no evidence of MET. Expressions of MMP1, MMP3, MMP7, and MMP26 were increased by aPRP (p < 0.05) in eMSC and eSF. Transcripts for inflammation markers/chemokines were upregulated by aPRP vs. aPPP (p < 0.05) in http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Assisted Reproduction and Genetics Springer Journals

In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration

14 pages
Read Article

Loading next page...
 
/lp/springer_journal/in-vitro-evidence-that-platelet-rich-plasma-stimulates-cellular-xuJBuaaiTp
Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Medicine & Public Health; Gynecology; Reproductive Medicine; Human Genetics
ISSN
1058-0468
eISSN
1573-7330
D.O.I.
10.1007/s10815-018-1130-8
Publisher site
See Article on Publisher Site

Abstract

Purpose The study aims to test the hypothesis that platelet-rich plasma (PRP) stimulates cellular processes involved in endome- trial regeneration relevant to clinical management of poor endometrial growth or intrauterine scarring. Methods Human endometrial stromal fibroblasts (eSF), endometrial mesenchymal stem cells (eMSC), bone marrow-derived mesenchymal stem cells (BM-MSC), and Ishikawa endometrial adenocarcinoma cells (IC) were cultured with/without 5% activated (a) PRP, non-activated (na) PRP, aPPP (platelet-poor-plasma), and naPPP. Treatment effects were evaluated with cell proliferation (WST-1), wound healing, and chemotaxis Transwell migration assays. Mesenchymal-to-epithelial transition (MET) was evaluated by cytokeratin and vimentin expression. Differential gene expression of various markers was analyzed by multiplex Q-PCR. Results Activated PRP enhanced migration of all cell types, compared to naPRP, aPPP, naPPP, and vehicle controls, in a time- dependent manner (p < 0.05). The WST-1 assay showed increased stromal and mesenchymal cell proliferation by aPRP vs. naPRP, aPPP, and naPPP (p < 0.05), while IC proliferation was enhanced by aPRP and aPPP (p < 0.05). There was no evidence of MET. Expressions of MMP1, MMP3, MMP7, and MMP26 were increased by aPRP (p < 0.05) in eMSC and eSF. Transcripts for inflammation markers/chemokines were upregulated by aPRP vs. aPPP (p < 0.05) in

Journal

Journal of Assisted Reproduction and Genetics – Springer Journals

Published: Feb 5, 2018

References

  • National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys
    Mascarenhas, MN; Flaxman, SR; Boerma, T; Vanderpoel, S; Stevens, GA
  • Uterine receptivity and the ramifications of ovarian stimulation on endometrial function
    Horcajadas, JA; Diaz-Gimeno, P; Pellicer, A; Simon, C
  • Endometrial receptivity is affected in women with high circulating progesterone levels at the end of the follicular phase: a functional genomics analysis
    Labarta, E; Martinez-Conejero, JA; Alama, P; Horcajadas, JA; Pellicer, A; Simon, C
  • Evidence that the endometrial microbiota has an effect on implantation success or failure
    Moreno, I; Codoner, FM; Vilella, F; Valbuena, D; Martinez-Blanch, JF; Jimenez-Almazan, J
  • Outcome of assisted reproduction treatment in patients with endometrial thickness less than 7 mm
    Kumbak, B; Erden, HF; Tosun, S; Akbas, H; Ulug, U; Bahceci, M
  • Endometrial thickness: a predictor of implantation in ovum recipients?
    Abdalla, HI; Brooks, AA; Johnson, MR; Kirkland, A; Thomas, A; Studd, JW
  • Determinants of pregnancy rate in the donor oocyte model: a multivariate analysis of 450 frozen-thawed embryo transfers
    Dessolle, L; Darai, E; Cornet, D; Rouzier, R; Coutant, C; Mandelbaum, J
  • Endometrial thickness and pregnancy rates after IVF: a systematic review and meta-analysis
    Kasius, A; Smit, JG; Torrance, HL; Eijkemans, MJ; Mol, BW; Opmeer, BC
  • Treating patients with “thin” endometrium—an ongoing challenge
    Lebovitz, O; Orvieto, R
  • Etiology of and therapeutic approach to synechia uteri
    Schenker, JG
  • Fertility after treatment of Asherman's syndrome stage 3 and 4
    Fernandez, H; Al-Najjar, F; Chauveaud-Lambling, A; Frydman, R; Gervaise, A
  • Endometrial regeneration using autologous adult stem cells followed by conception by in vitro fertilization in a patient of severe Asherman’s syndrome
    Nagori, CB; Panchal, SY; Patel, H
  • Human amnion as a temporary biologic barrier after hysteroscopic lysis of severe intrauterine adhesions: pilot study
    Amer, MI; Abd-El-Maeboud, KH; Abdelfatah, I; Salama, FA; Abdallah, AS
  • Autologous cell therapy with CD133+ bone marrow-derived stem cells for refractory Asherman’s syndrome and endometrial atrophy: a pilot cohort study
    Santamaria, X; Cabanillas, S; Cervello, I; Arbona, C; Raga, F; Ferro, J
  • Extended estrogen administration for women with thin endometrium in frozen-thawed in-vitro fertilization programs
    Chen, MJ; Yang, JH; Peng, FH; Chen, SU; Ho, HN; Yang, YS
  • Low-dose aspirin for oocyte donation recipients with a thin endometrium: prospective, randomized study
    Weckstein, LN; Jacobson, A; Galen, D; Hampton, K; Hammel, J
  • Effect of vaginal sildenafil on the outcome of in vitro fertilization (IVF) after multiple IVF failures attributed to poor endometrial development
    Sher, G; Fisch, JD
  • A pilot cohort study of granulocyte colony-stimulating factor in the treatment of unresponsive thin endometrium resistant to standard therapies
    Gleicher, N; Kim, A; Michaeli, T; Lee, HJ; Shohat-Tal, A; Lazzaroni, E
  • Autologous platelet-rich plasma promotes endometrial growth and improves pregnancy outcome during in vitro fertilization
    Chang, Y; Li, J; Chen, Y; Wei, L; Yang, X; Shi, Y
  • Treatment of thin endometrium with autologous platelet-rich plasma: a pilot study
    Zadehmodarres, S; Salehpour, S; Saharkhiz, N; Nazari, L
  • Platelet-rich plasma as a treatment for patellar tendinopathy: a double-blind, randomized controlled trial
    Dragoo, JL; Wasterlain, AS; Braun, HJ; Nead, KT
  • Platelet-rich plasma for arthroscopic repair of large to massive rotator cuff tears: a randomized, single-blind, parallel-group trial
    Jo, CH; Shin, JS; Lee, YG; Shin, WH; Kim, H; Lee, SY
  • Application of platelet-rich plasma in plastic surgery: clinical and in vitro evaluation
    Cervelli, V; Gentile, P; Scioli, MG; Grimaldi, M; Casciani, CU; Spagnoli, LG
  • The growing evidence for the use of platelet-rich plasma on diabetic chronic wounds: a review and a proposal for a new standard care
    Picard, F; Hersant, B; Bosc, R; Meningaud, JP
  • Platelet derived bFGF mediates vascular integrative mechanisms of mesenchymal stem cells in vitro
    Langer, HF; Stellos, K; Steingen, C; Froihofer, A; Schonberger, T; Kramer, B
  • Platelet aggregates-induced human CD34+ progenitor cell proliferation and differentiation to macrophages and foam cells is mediated by stromal cell derived factor 1 in vitro
    Stellos, K; Seizer, P; Bigalke, B; Daub, K; Geisler, T; Gawaz, M
  • Hormone and growth factor signaling in endometrial renewal: role of stem/progenitor cells
    Gargett, CE; Chan, RW; Schwab, KE
  • Regulation of proliferation, motility, and contractility of human endometrial stromal cells by platelet-derived growth factor
    Matsumoto, H; Nasu, K; Nishida, M; Ito, H; Bing, S; Miyakawa, I
  • Adult stem cells in the endometrium
    Gargett, CE; Masuda, H
  • Identification of label-retaining perivascular cells in a mouse model of endometrial decidualization, breakdown, and repair
    Kaitu'u-Lino, TJ; Ye, L; Salamonsen, LA; Girling, JE; Gargett, CE
  • Contribution of bone marrow-derived stem cells to endometrium and endometriosis
    Du, H; Taylor, HS
  • The bone marrow-derived human mesenchymal stem cell: potential progenitor of the endometrial stromal fibroblast
    Aghajanova, L; Horcajadas, JA; Esteban, FJ; Giudice, LC
  • Bone marrow-derived cells from male donors can compose endometrial glands in female transplant recipients
    Ikoma, T; Kyo, S; Maida, Y; Ozaki, S; Takakura, M; Nakao, S
  • Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts
    Kakudo, N; Minakata, T; Mitsui, T; Kushida, S; Notodihardjo, FZ; Kusumoto, K
  • Steroidogenic enzyme and key decidualization marker dysregulation in endometrial stromal cells from women with versus without endometriosis
    Aghajanova, L; Hamilton, A; Kwintkiewicz, J; Vo, KC; Giudice, LC
  • The protein kinase a pathway-regulated transcriptome of endometrial stromal fibroblasts reveals compromised differentiation and persistent proliferative potential in endometriosis
    Aghajanova, L; Horcajadas, JA; Weeks, JL; Esteban, FJ; Nezhat, CN; Conti, M
  • Cryopreservation and recovery of human endometrial epithelial cells with high viability, purity, and functional fidelity
    Chen, JC; Hoffman, JR; Arora, R; Perrone, LA; Gonzalez-Gomez, CJ; Vo, KC
  • Hormonal regulation of human endometrial stromal cells in culture: an in vitro model for decidualization
    Irwin, JC; Kirk, D; King, RJ; Quigley, MM; Gwatkin, RB
  • Perivascular human endometrial mesenchymal stem cells express pathways relevant to self-renewal, lineage specification, and functional phenotype
    Spitzer, TL; Rojas, A; Zelenko, Z; Aghajanova, L; Erikson, DW; Barragan, F
  • Human endometrial fibroblasts derived from mesenchymal progenitors inherit progesterone resistance and acquire an inflammatory phenotype in the endometrial niche in endometriosis
    Barragan, F; Irwin, JC; Balayan, S; Erikson, DW; Chen, JC; Houshdaran, S
  • Inhibition of transforming growth factor-beta receptor signaling promotes culture expansion of undifferentiated human endometrial mesenchymal stem/stromal cells
    Gurung, S; Werkmeister, JA; Gargett, CE
  • Thyroid-stimulating hormone receptor and thyroid hormone receptors are involved in human endometrial physiology
    Aghajanova, L; Stavreus-Evers, A; Lindeberg, M; Landgren, BM; Sparre, LS; Hovatta, O
  • Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells
    Cho, HS; Song, IH; Park, SY; Sung, MC; Ahn, MW; Song, KE
  • Can platelet-rich plasma be used for skin rejuvenation? Evaluation of effects of platelet-rich plasma on human dermal fibroblast
    Kim, DH; Je, YJ; Kim, CD; Lee, YH; Seo, YJ; Lee, JH
  • Effects of platelet-rich plasma on proliferation and myofibroblastic differentiation in human dermal fibroblasts
    Kushida, S; Kakudo, N; Suzuki, K; Kusumoto, K
  • Cell migration and invasion assays as tools for drug discovery
    Hulkower, KI; Herber, RL
  • Suppression of the epithelial-mesenchymal transition by SHARP1 is linked to the NOTCH1 signaling pathway in metastasis of endometrial cancer
    Liao, Y; He, X; Qiu, H; Che, Q; Wang, F; Lu, W
  • Coculturing human endometrial epithelial cells and stromal fibroblasts alters cell-specific gene expression and cytokine production
    Chen, JC; Erikson, DW; Piltonen, TT; Meyer, MR; Barragan, F; McIntire, RH
  • Molecular classification of endometriosis and disease stage using high-dimensional genomic data
    Tamaresis, JS; Irwin, JC; Goldfien, GA; Rabban, JT; Burney, RO; Nezhat, C
  • Mesenchymal-to-epithelial transition contributes to endometrial regeneration following natural and artificial decidualization
    Patterson, AL; Zhang, L; Arango, NA; Teixeira, J; Pru, JK
  • Evidence from a mouse model that epithelial cell migration and mesenchymal-epithelial transition contribute to rapid restoration of uterine tissue integrity during menstruation
    Cousins, FL; Murray, A; Esnal, A; Gibson, DA; Critchley, HO; Saunders, PT
  • Regenerating endometrium from stem/progenitor cells: is it abnormal in endometriosis, Asherman’s syndrome and infertility?
    Deane, JA; Gualano, RC; Gargett, CE
  • Wound repair and regeneration
    Gurtner, GC; Werner, S; Barrandon, Y; Longaker, MT
  • Platelet-rich plasma: quantification of growth factor levels and the effect on growth and differentiation of rat bone marrow cells
    Dolder, J; Mooren, R; Vloon, AP; Stoelinga, PJ; Jansen, JA
  • The effect of platelet-rich plasma on the differentiation of synovium-derived mesenchymal stem cells
    Lee, JK; Lee, S; Han, SA; Seong, SC; Lee, MC
  • Effect of autologous and allogenic platelet-rich plasma on human gingival fibroblast function
    Creeper, F; Ivanovski, S
  • Transgelins, cytoskeletal proteins implicated in different aspects of cancer development
    Dvorakova, M; Nenutil, R; Bouchal, P
  • TAGLN expression is deregulated in endometriosis and may be involved in cell invasion, migration, and differentiation
    Santos Hidalgo, G; Meola, J; Rosa, ESJC; Paro de Paz, CC; Ferriani, RA
  • Platelets drive smooth muscle metaplasia and fibrogenesis in endometriosis through epithelial-mesenchymal transition and fibroblast-to-myofibroblast transdifferentiation
    Zhang, Q; Duan, J; Liu, X; Guo, SW
  • Corroborating evidence for platelet-induced epithelial-mesenchymal transition and fibroblast-to-myofibroblast transdifferentiation in the development of adenomyosis
    Liu, X; Shen, M; Qi, Q; Zhang, H; Guo, SW
  • Anti-platelet therapy holds promises in treating adenomyosis: experimental evidence
    Zhu, B; Chen, Y; Shen, X; Liu, X; Guo, SW
  • Progressive development of endometriosis and its hindrance by anti-platelet treatment in mice with induced endometriosis
    Zhang, Q; Liu, X; Guo, SW
  • Global transcriptome abnormalities of the eutopic endometrium from women with adenomyosis
    Herndon, CN; Aghajanova, L; Balayan, S; Erikson, D; Barragan, F; Goldfien, G
  • Platelet-rich plasma attenuates 30-kDa fibronectin fragment-induced chemokine and matrix metalloproteinase expression by meniscocytes and articular chondrocytes
    Wang, CC; Lee, CH; Peng, YJ; Salter, DM; Lee, HS
  • Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro
    Marini, MG; Perrini, C; Esposti, P; Corradetti, B; Bizzaro, D; Riccaboni, P
  • Inflammatory response in chronic degenerative endometritis mares treated with platelet-rich plasma
    Reghini, MF; Ramires Neto, C; Segabinazzi, LG; Castro Chaves, MM; Dell’Aqua Cde, P; Bussiere, MC
  • The basic science of wound healing
    Broughton, G; Janis, JE; Attinger, CE
  • Stromal-to-epithelial transition during postpartum endometrial regeneration
    Huang, CC; Orvis, GD; Wang, Y; Behringer, RR
  • Metalloproteinases and wound healing
    Caley, MP; Martins, VL; O'Toole, EA
  • Regulation of matrix metalloproteinase gene expression
    Yan, C; Boyd, DD
  • Can platelet-rich plasma enhance tendon repair? A cell culture study
    Mos, M; Windt, AE; Jahr, H; Schie, HT; Weinans, H; Verhaar, JA
  • The effects of platelet-rich clot releasate on the expression of MMP-1 and type I collagen in human adult dermal fibroblasts: PRP is a stronger MMP-1 stimulator
    Shin, MK; Lee, JW; Kim, YI; Kim, YO; Seok, H; Kim, NI
  • Platelet concentrate in bovine reproduction: effects on in vitro embryo production and after intrauterine administration in repeat breeder cows
    Lange-Consiglio, A; Cazzaniga, N; Garlappi, R; Spelta, C; Pollera, C; Perrini, C
  • Activated platelets induce estrogen receptor beta expression in endometriotic stromal cells
    Zhang, Q; Ding, D; Liu, X; Guo, SW

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial