In vitro binding and bimolecular fluorescence complementation assays suggest an interaction between tomato mosaic virus coat protein and tobacco chloroplast ferredoxin I

In vitro binding and bimolecular fluorescence complementation assays suggest an interaction... Symptom development of a viral plant disease results from molecular interactions between the virus and its host plant. Tomato mosaic virus coat protein (ToMV CP) not only plays a major role in virion assembly and long-distance movement but is also responsible for symptom development in ToMV-infected plants. This study provides evidence that chloroplast ferredoxin I (Fd I) interacts with ToMV CP in a GAL4-based two-hybrid yeast system for screening a Nicotiana tabacum cDNA library. The interaction between CP and Fd I was confirmed by in vitro binding and bimolecular fluorescence complementation assays in plant cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

In vitro binding and bimolecular fluorescence complementation assays suggest an interaction between tomato mosaic virus coat protein and tobacco chloroplast ferredoxin I

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1778-4
Publisher site
See Article on Publisher Site

Abstract

Symptom development of a viral plant disease results from molecular interactions between the virus and its host plant. Tomato mosaic virus coat protein (ToMV CP) not only plays a major role in virion assembly and long-distance movement but is also responsible for symptom development in ToMV-infected plants. This study provides evidence that chloroplast ferredoxin I (Fd I) interacts with ToMV CP in a GAL4-based two-hybrid yeast system for screening a Nicotiana tabacum cDNA library. The interaction between CP and Fd I was confirmed by in vitro binding and bimolecular fluorescence complementation assays in plant cells.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2013

References

  • Influence of host chloroplast proteins on Tobacco mosaic virus accumulation and intercellular movement
    Bhat, S; Folimonova, SY; Cole, AB; Ballard, KD; Lei, Z; Watson, BS; Sumner, LW; Nelson, RS
  • Identification of a 37 kDa plant protein that interacts with the turnip mosaic potyvirus capsid protein using anti-idiotypic-antibodies
    McClintock, K; Lamarre, A; Parsons, V; Laliberte, JF; Fortin, MG

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