In Vitro Apoptosis Induction in a Human Prostate Cancer Cell Line by Thermotolerant Glycolipid from Bacillus licheniformis SV1

In Vitro Apoptosis Induction in a Human Prostate Cancer Cell Line by Thermotolerant Glycolipid... A highly potent glycolipid was isolated from a crude oil-contaminated soil bacterium Bacillus licheniformis SV1 (NCBI GenBank Acc. No. KX130852) when grown on a modified mineral salt medium supplemented with 2% oleic acid. The maximum reduction in surface tension of cell-free broth from 71 ± 0.812 to 25.919 ± 0.984 mN/m with 89 ± 0.346% emulsification activity was recorded after 120 h of growth. Glycolipid was purified using chromatographic techniques and the presence of aliphatic chain (C–H stretch) and OH-band was revealed by NMR, GC–MS and FTIR analysis. Stability of glycolipid up to 250 °C and its complete decomposition at 507 °C was recorded by thermogravimetric analysis (TGA). MTT assay (IC50 = 0.473 ± 0.048 mg/ml) along with 4′,6-diamidino-2-phenylindole (DAPI) and acridine orange and ethidium bromide (AO/EtBr) staining validated that glycolipid induces cell apoptosis in human prostate cancer cell line (PC-3). Furthermore, potent anti-cancerous compounds such as 9-octadecanoic acid (22.55%), linoleic acid methyl ester (2.2%) and palmitic acid (1.18%) were also detected in the GC–MS spectra of the purified glycolipid. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Surfactants and Detergents Springer Journals

In Vitro Apoptosis Induction in a Human Prostate Cancer Cell Line by Thermotolerant Glycolipid from Bacillus licheniformis SV1

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by AOCS
Subject
Chemistry; Industrial Chemistry/Chemical Engineering; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution; Surfaces and Interfaces, Thin Films; Polymer Sciences; Physical Chemistry
ISSN
1097-3958
eISSN
1558-9293
D.O.I.
10.1007/s11743-017-1986-0
Publisher site
See Article on Publisher Site

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