In silico Search for the Expressed Sequences in the Region q14.3 of Human Chromosome 13

In silico Search for the Expressed Sequences in the Region q14.3 of Human Chromosome 13 Using computer-aided genomic methods, a complete map of the expressed sequence tags (EST) located in the human genome region 13q14.3 between the STS markers, D13S810 and D13S1469, was constructed. A total of 62 EST clusters were formed, of which 12 clusters corresponded to the already known human genes, 4 clusters represented pseudogenes, and 10 clusters were new human genes. The use of the method of reverse transcription in combination with polymerase chain reaction (RT–PCR) provided experimental confirmation of the existence of mRNA transcripts for the novel human genes revealed in silico. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

In silico Search for the Expressed Sequences in the Region q14.3 of Human Chromosome 13

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2004 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/B:RUGE.0000021636.40144.5c
Publisher site
See Article on Publisher Site

Abstract

Using computer-aided genomic methods, a complete map of the expressed sequence tags (EST) located in the human genome region 13q14.3 between the STS markers, D13S810 and D13S1469, was constructed. A total of 62 EST clusters were formed, of which 12 clusters corresponded to the already known human genes, 4 clusters represented pseudogenes, and 10 clusters were new human genes. The use of the method of reverse transcription in combination with polymerase chain reaction (RT–PCR) provided experimental confirmation of the existence of mRNA transcripts for the novel human genes revealed in silico.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 18, 2004

References

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