Impedimetric immunoassay for aflatoxin B1 using a cysteine modified gold electrode with covalently immobilized carbon nanotubes

Impedimetric immunoassay for aflatoxin B1 using a cysteine modified gold electrode with... The authors describe a label-free electrochemical biosensor based on CNTs for picogram range detection of the food carcinogen aflatoxin B1 (AFB1). A gold electrode was modified with a cysteine (Cys) self-assembled layer, and carboxyl-functionalized carbon nanotubes were covalently attached to Cys for the subsequent tethering of antibody against AFB1 (anti-AFB1). Topographical images of the biosensor surface were acquired by atomic force microscopy throughout the modification and assay procedure. Upon exposure to samples containing AFB1, its binding to anti-AFB1 will result in a change in electrical conductivity. The use of CNT warrants enhanced electrical properties, and the charge-transfer resistance (Rct) can be related to the concentration of AFB1. Biosensor selectivity was tested by using samples containing ochratoxin A. The electrode displays a limit of detection as low as 0.79 pg·g−1, and response is linear in the 0.1 to 20 pg·g−1 concentration range. The assay was applied to the determination of AFB1 in contaminated corn flour at concentrations so low that they cannot be quantified by established ELISAs. In our perception, this method represents a viable point-of-care probe for detection of AFB1. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microchimica Acta Springer Journals

Impedimetric immunoassay for aflatoxin B1 using a cysteine modified gold electrode with covalently immobilized carbon nanotubes

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Publisher
Springer Vienna
Copyright
Copyright © 2017 by Springer-Verlag Wien
Subject
Chemistry; Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering
ISSN
0026-3672
eISSN
1436-5073
D.O.I.
10.1007/s00604-017-2308-y
Publisher site
See Article on Publisher Site

Abstract

The authors describe a label-free electrochemical biosensor based on CNTs for picogram range detection of the food carcinogen aflatoxin B1 (AFB1). A gold electrode was modified with a cysteine (Cys) self-assembled layer, and carboxyl-functionalized carbon nanotubes were covalently attached to Cys for the subsequent tethering of antibody against AFB1 (anti-AFB1). Topographical images of the biosensor surface were acquired by atomic force microscopy throughout the modification and assay procedure. Upon exposure to samples containing AFB1, its binding to anti-AFB1 will result in a change in electrical conductivity. The use of CNT warrants enhanced electrical properties, and the charge-transfer resistance (Rct) can be related to the concentration of AFB1. Biosensor selectivity was tested by using samples containing ochratoxin A. The electrode displays a limit of detection as low as 0.79 pg·g−1, and response is linear in the 0.1 to 20 pg·g−1 concentration range. The assay was applied to the determination of AFB1 in contaminated corn flour at concentrations so low that they cannot be quantified by established ELISAs. In our perception, this method represents a viable point-of-care probe for detection of AFB1.

Journal

Microchimica ActaSpringer Journals

Published: May 25, 2017

References

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