Impedimetric detection of cocaine by using an aptamer attached to a screen printed electrode modified with a dendrimer/silver nanoparticle nanocomposite

Impedimetric detection of cocaine by using an aptamer attached to a screen printed electrode... The authors describe a highly sensitive method for the aptamer (Apt) based impedimetric determination of cocaine. The surface of a screen-printed electrode (SPE) was modified with a nanocomposite of dendrimer and silver nanoparticles (AgNPs). The cocaine-binding Apt was attached to a dendrimer/AgNP/SPE surface, forming a sensitive layer for the determination of cocaine. The incubation with the analyte resulted in the formation of a cocaine/Apt complex on the electrode surface. As a consequence, folding and conformational change in the aptamer structure was induced, this resulting in a change in the impedimetric signal. The aptaassay exhibits highly efficient sensing characteristics with a good linearity of 1 fmol L−1 to 100 nmol L−1 (with two linear ranges) and a limit of detection (LOD) of 333 amol L−1. Its excellent specificity and high sensitivity suggest that this kind of aptamer-based assay may be applied to detect other targets in this field. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microchimica Acta Springer Journals

Impedimetric detection of cocaine by using an aptamer attached to a screen printed electrode modified with a dendrimer/silver nanoparticle nanocomposite

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Publisher
Springer Vienna
Copyright
Copyright © 2018 by Springer-Verlag GmbH Austria, part of Springer Nature
Subject
Chemistry; Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering
ISSN
0026-3672
eISSN
1436-5073
D.O.I.
10.1007/s00604-018-2709-6
Publisher site
See Article on Publisher Site

Abstract

The authors describe a highly sensitive method for the aptamer (Apt) based impedimetric determination of cocaine. The surface of a screen-printed electrode (SPE) was modified with a nanocomposite of dendrimer and silver nanoparticles (AgNPs). The cocaine-binding Apt was attached to a dendrimer/AgNP/SPE surface, forming a sensitive layer for the determination of cocaine. The incubation with the analyte resulted in the formation of a cocaine/Apt complex on the electrode surface. As a consequence, folding and conformational change in the aptamer structure was induced, this resulting in a change in the impedimetric signal. The aptaassay exhibits highly efficient sensing characteristics with a good linearity of 1 fmol L−1 to 100 nmol L−1 (with two linear ranges) and a limit of detection (LOD) of 333 amol L−1. Its excellent specificity and high sensitivity suggest that this kind of aptamer-based assay may be applied to detect other targets in this field.

Journal

Microchimica ActaSpringer Journals

Published: Mar 12, 2018

References

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