Impact on antibody responses of B-cell-restricted transgenic expression of a viral gene inhibiting activation of NF-κB and NFAT

Impact on antibody responses of B-cell-restricted transgenic expression of a viral gene... In this work, we have assessed the impact in vivo of the evasion gene A238L of African swine fever virus, an inhibitor of both NF-κB- and NFAT-mediated transcription. The A238L gene was selectively expressed in mouse B lymphocytes using the promoter and enhancer sequences of the mouse Ig μ heavy chain. The IgM primary and IgG2b secondary serological responses and the number of splenic germinal centres in response to the TD antigens DNP-keyhole limpet hemocyanin and sheep red blood cells, respectively, were both lower in the transgenic mice, whereas the response to the TI type-1 and type-2 antigens DNP-Ficoll and DNP-LPS, respectively, were normal, except for the increased levels of IgG3 at day 14 in the DNP-LPS-immunized mice. Thus, it appears that neither p65 (NF-κB) nor NFAT is essential for B-cell development but, in a manner that is still unclear, may be relevant for their function. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Impact on antibody responses of B-cell-restricted transgenic expression of a viral gene inhibiting activation of NF-κB and NFAT

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2419-x
Publisher site
See Article on Publisher Site

References

  • Mechanism and control of V(D)J recombination at the immunoglobulin heavy chain locus
    Jung, D; Giallourakis, C; Mostoslavsky, R; Alt, FW
  • Two distinct action mechanisms of immunophilin-ligand complexes for the blockade of T-cell activation
    Matsuda, S; Shibasaki, F; Takehana, K; Mori, H; Nishida, E; Koyasu, S
  • Retarded thymic involution and massive germinal center formation in NF-ATp-deficient mice
    Schuh, K; Kneitz, B; Heyer, J; Bommhardt, U; Jankevics, E; Berberich-Siebelt, F

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