Impact of the Regulatory Regions of Retrotransposon copia on the Level of Its Expression in Testes of Drosophila melanogaster

Impact of the Regulatory Regions of Retrotransposon copia on the Level of Its Expression in... Expression of the lacZ reporter gene under the control of five deletion derivatives of the copia regulatory region including the 5′ long terminal repeat (LTR) and the 5′ untranslated region (UTR) was assayed in the testes of transgenic Drosophila melanogaster males (larvae and imago). The full-length copia regulatory region (LTR + UTR) ensured expression of the reporter gene in testes of both larvae and adult males. Deletion of UTR or 3′ end of LTR increased lacZ expression in the testes, whereas deletion of the 5′ end of LTR increased it. This indicated that a positive regulator ofcopia expression is at the 5′ end of LTR and that negative regulators are at the 3′ end of LTR and in UTR. The effects of the fragments of the copia regulatory region on reporter gene expression in the testes in vivo did not completely coincide with the effects observed earlier in cultured cells. We suggest that this difference is due to different regulation of expression of the fusion constructs integrated into chromatin as compared to their transient expression. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Impact of the Regulatory Regions of Retrotransposon copia on the Level of Its Expression in Testes of Drosophila melanogaster

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Publisher
Kluwer Academic Publishers-Plenum Publishers
Copyright
Copyright © 2004 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/B:RUGE.0000016984.82723.d3
Publisher site
See Article on Publisher Site

Abstract

Expression of the lacZ reporter gene under the control of five deletion derivatives of the copia regulatory region including the 5′ long terminal repeat (LTR) and the 5′ untranslated region (UTR) was assayed in the testes of transgenic Drosophila melanogaster males (larvae and imago). The full-length copia regulatory region (LTR + UTR) ensured expression of the reporter gene in testes of both larvae and adult males. Deletion of UTR or 3′ end of LTR increased lacZ expression in the testes, whereas deletion of the 5′ end of LTR increased it. This indicated that a positive regulator ofcopia expression is at the 5′ end of LTR and that negative regulators are at the 3′ end of LTR and in UTR. The effects of the fragments of the copia regulatory region on reporter gene expression in the testes in vivo did not completely coincide with the effects observed earlier in cultured cells. We suggest that this difference is due to different regulation of expression of the fusion constructs integrated into chromatin as compared to their transient expression.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 18, 2004

References

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