Immunoprecipitation mapping of TRX-associated chromosome elements in the fork head gene promoter in Drosophila melanogaster salivary gland cells

Immunoprecipitation mapping of TRX-associated chromosome elements in the fork head gene promoter... Using the method of immunoprecipitation of the in vivo crosslinked and sheared by sonication chromatin, mapping of potential trithorax-associated regulatory elements within the extended (9 kb) promoter region of the fork head gene (fkh) in the Drosophila melanogaster salivary gland cells was performed. Reaative homogeneity of the salivary gland cells, along with the parallel use of the antibodies to different domains of the same trithorax protein (TRX), and the introduction of cross-hybridization steps for additional specific enrichment of initial DNA libraries, allowed us to improve the method and to identify one major and two less evident potential TRX-binding sites. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Immunoprecipitation mapping of TRX-associated chromosome elements in the fork head gene promoter in Drosophila melanogaster salivary gland cells

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2007 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795407090037
Publisher site
See Article on Publisher Site

Abstract

Using the method of immunoprecipitation of the in vivo crosslinked and sheared by sonication chromatin, mapping of potential trithorax-associated regulatory elements within the extended (9 kb) promoter region of the fork head gene (fkh) in the Drosophila melanogaster salivary gland cells was performed. Reaative homogeneity of the salivary gland cells, along with the parallel use of the antibodies to different domains of the same trithorax protein (TRX), and the introduction of cross-hybridization steps for additional specific enrichment of initial DNA libraries, allowed us to improve the method and to identify one major and two less evident potential TRX-binding sites.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Sep 27, 2007

References

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