Imaging diffusion in a microfluidic device by third harmonic microscopy

Imaging diffusion in a microfluidic device by third harmonic microscopy We monitor and characterize near-surface diffusion of miscible, transparent liquids in a microfluidic device by third harmonic microscopy. The technique enables observations even of transparent or index-matched media without perturbation of the sample. In particular, we image concentrations of ethanol diffusing in water and estimate the diffusion coefficient from the third harmonic images. We obtain a diffusion coefficient D = (460 ± 30) μm2/s, which is consistent with theoretical predictions. The investigations clearly demonstrate the potential of harmonic microscopy also under the challenging conditions of transparent fluids. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Experiments in Fluids Springer Journals

Imaging diffusion in a microfluidic device by third harmonic microscopy

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Publisher
Springer-Verlag
Copyright
Copyright © 2012 by Springer-Verlag
Subject
Engineering; Engineering Thermodynamics, Heat and Mass Transfer; Engineering Fluid Dynamics; Fluid- and Aerodynamics
ISSN
0723-4864
eISSN
1432-1114
D.O.I.
10.1007/s00348-012-1321-5
Publisher site
See Article on Publisher Site

Abstract

We monitor and characterize near-surface diffusion of miscible, transparent liquids in a microfluidic device by third harmonic microscopy. The technique enables observations even of transparent or index-matched media without perturbation of the sample. In particular, we image concentrations of ethanol diffusing in water and estimate the diffusion coefficient from the third harmonic images. We obtain a diffusion coefficient D = (460 ± 30) μm2/s, which is consistent with theoretical predictions. The investigations clearly demonstrate the potential of harmonic microscopy also under the challenging conditions of transparent fluids.

Journal

Experiments in FluidsSpringer Journals

Published: Jun 6, 2012

References

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