IL-17A suppresses and curcumin up-regulates Akt expression upon bleomycin exposure

IL-17A suppresses and curcumin up-regulates Akt expression upon bleomycin exposure Pro-inflammatory cytokine IL-17A modulates the expression of Akt in bleomycin (BLM) administered alveolar basal epi- thelial cells, the mechanism behind which remains unclear. This investigation was carried out to assess IL-17A mediated down-regulation of Akt expression and the pivotal role of curcumin as a regulatory molecule. Alveolar basal epithelial cells were treated with BLM and IL-17A and curcumin was administered as an intervention to regulate the BLM-induced oxidative damage. Cell proliferation was evaluated by clonogenic assay. Akt phosphorylation and total Akt expressions were studied using western blot analysis. Cell proliferation reduced upon treatment with BLM and this phenomenon was reversed in cells upon administration with curcumin. Administrations of BLM and IL-17A to the alveolar basal epithelial cells showed significant down-regulation of Akt expression which was reversed by treatment with curcumin. BLM and IL-17A mediated inflammation was intervened effectively with curcumin. Results of this study suggest the probable use of curcumin as an anti-inflammatory therapeutic for lung injury. Keywords Bleomycin · IL-17A · Curcumin · Akt · Inflammation Introduction Bleomycin (BLM) mediated inflammatory pathway plays a major role in the AEC apoptosis and its viability Acute lung inflammation is distinguished by infiltration of [4]. Action of BLM is mediated through the pro-inflamma- alveolar macrophages, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Biology Reports Springer Journals

IL-17A suppresses and curcumin up-regulates Akt expression upon bleomycin exposure

Loading next page...
 
/lp/springer_journal/il-17a-suppresses-and-curcumin-up-regulates-akt-expression-upon-CQHOuB7Zts
Publisher
Springer Netherlands
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Life Sciences; Animal Biochemistry; Animal Anatomy / Morphology / Histology
ISSN
0301-4851
eISSN
1573-4978
D.O.I.
10.1007/s11033-018-4199-3
Publisher site
See Article on Publisher Site

Abstract

Pro-inflammatory cytokine IL-17A modulates the expression of Akt in bleomycin (BLM) administered alveolar basal epi- thelial cells, the mechanism behind which remains unclear. This investigation was carried out to assess IL-17A mediated down-regulation of Akt expression and the pivotal role of curcumin as a regulatory molecule. Alveolar basal epithelial cells were treated with BLM and IL-17A and curcumin was administered as an intervention to regulate the BLM-induced oxidative damage. Cell proliferation was evaluated by clonogenic assay. Akt phosphorylation and total Akt expressions were studied using western blot analysis. Cell proliferation reduced upon treatment with BLM and this phenomenon was reversed in cells upon administration with curcumin. Administrations of BLM and IL-17A to the alveolar basal epithelial cells showed significant down-regulation of Akt expression which was reversed by treatment with curcumin. BLM and IL-17A mediated inflammation was intervened effectively with curcumin. Results of this study suggest the probable use of curcumin as an anti-inflammatory therapeutic for lung injury. Keywords Bleomycin · IL-17A · Curcumin · Akt · Inflammation Introduction Bleomycin (BLM) mediated inflammatory pathway plays a major role in the AEC apoptosis and its viability Acute lung inflammation is distinguished by infiltration of [4]. Action of BLM is mediated through the pro-inflamma- alveolar macrophages,

Journal

Molecular Biology ReportsSpringer Journals

Published: May 28, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off